Interferon-related gene array in predicting the efficacy of interferon therapy in chronic hepatitis B

• Published in: Sheng wu yi xue gong cheng xue za zhi 2023-02, Vol.40 (1), p.79-86
• Main Authors: Wang, Jiayi, Lu, Jiajie, Zhou, Chen, Du, Lingyao, Tang, Hong
• Format: Article
• Language: Chinese
• Subjects: Arrays; CXCL10 protein; Effectiveness; Gene expression; Genes; Healthy Volunteers; Hepatitis; Hepatitis B; Hepatitis B, Chronic - drug therapy; Hepatitis B, Chronic - genetics; Humans; Immunotherapy; Interferon; Interferon regulatory factor 1; Interferon regulatory factor 3; Interferon regulatory factor 4; Interferons - therapeutic use; Interleukin 15; Interleukin 4 receptors; Interleukin 7 receptors; Leukocytes, Mononuclear; Monocytes; Nuclear Proteins; Oligonucleotide Array Sequence Analysis - methods; Peripheral blood mononuclear cells; Treatment Outcome; α-Interferon
• ISSN: 1001-5515
• DOI: 10.7507/1001-5515.202301014

This study aims to clarify host factors of IFN treatment in the treatment of chronic hepatitis B (CHB) patients by screening the differentially expressed genes of IFN pathway CHB patients with different response to interferon (IFN) therapy. Three cases were randomly selected in IFN-responding CHB patients (Rs), non-responding CHB patients (NRs) and healthy participants, respectively. The human type I IFN response RT profiler PCR array was used to detect the expression levels of IFN-related genes in peripheral blood monocytes (PBMCs) from healthy participants and CHB patients before and after Peg-IFN-α 2a treatment. The results showed that more differentially expressed genes appeared in Rs group than NRs group after IFN treatment. Comparing with healthy participants, IFNG, IL7R, IRF1, and IRF8 were downregulated in both Rs and NRs group before IFN treatment; CXCL10, IFIT1, and IFITM1 were upregulated in the Rs; IL13RA1 and IFI35 were upregulated in the NRs, while IFRD2, IL11RA, IL4R, IRF3, IRF4, PYHIN1, and AD