MALDI Imaging Assisted Discovery of a Di‐O‐glycosyltransferase from Platycodon grandiflorum Root

A matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) assisted genome mining strategy was developed for the discovery of glycosyltransferase (GT) from the root of Platycodon grandiflorum. A di‐O‐glycosyltransferase PgGT1 was discovered and characterized that is capable...

Full description

Saved in:
Bibliographic Details
Published in:Angewandte Chemie International Edition 2023-05, Vol.62 (19), p.e202301309-n/a
Main Authors: Tang, Weiwei, Shi, Jun‐Jie, Liu, Wei, Lu, Xu, Li, Bin
Format: Article
Language:English
Subjects:
Biotransformation
Chemical synthesis
Enzyme Catalysis
Genomes
Glucose
Glucose - metabolism
Glycosylation
Glycosyltransferase
Glycosyltransferases - metabolism
Ionization
MALDI Mass Spectrometry Imaging
Mass Spectrometry
Mass spectroscopy
N-Acetylglucosamine
Platycodon - chemistry
Platycodon - metabolism
Platycodon grandiflorum
Residues
Saccharides
Spatial Transcriptome
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Uridine Diphosphate - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) assisted genome mining strategy was developed for the discovery of glycosyltransferase (GT) from the root of Platycodon grandiflorum. A di‐O‐glycosyltransferase PgGT1 was discovered and characterized that is capable of catalyzing platycoside E (PE) synthesis through the attachment of two β‐1,6‐linked glucosyl residues sequentially to the glucosyl residue at the C3 position of platycodin D (PD). Although UDP‐glucose is the preferred sugar donor for PgGT1, it could also utilize UDP‐xylose and UDP‐N‐acetylglucosamine as weak donors. Residues S273, E274, and H350 played important roles in stabilizing the glucose donor and positioning the glucose in the optimal orientation for the glycosylation reaction. This study clarified two key steps involved in the biosynthetic pathway of PE and could greatly contribute to improving its industrial biotransformation. A MALDI MS imaging assisted genome screening strategy was developed and enabled the identification and characterization of a di‐O‐glycosyltransferase PgGT1 from Platycodon grandiflorum root.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.202301309