Influence of a hyaluronan-binding system for sperm selection in intracytoplasmic sperm injection cycles on embryo morphokinetic parameters and in vitro fertilization cycle outcomes

• Published in: Archives of gynecology and obstetrics 2023-05, Vol.307 (5), p.1633-1639
• Main Authors: Emirdar, Volkan, Karatasli, Volkan, Tamer, Burcu, Pala, Ibrahim, Gunturkun, Fatma, Ozbaykus, Canberk, Işık, Ahmet Zeki, Gode, Funda
• Format: Article
• Language: English
• Subjects: Abortion, Spontaneous; Embryos; Endocrinology; Female; Fertilization in Vitro - methods; Gynecologic Endocrinology and Reproductive Medicine; Gynecology; Human Genetics; Humans; Hyaluronic Acid; In vitro fertilization; Male; Medicine; Medicine & Public Health; Obstetrics/Perinatology/Midwifery; Pregnancy; Pregnancy Rate; Retrospective Studies; Semen; Sperm; Sperm Injections, Intracytoplasmic - methods; Spermatozoa - metabolism
• ISSN: 1432-0711; 0932-0067; 1432-0711
• DOI: 10.1007/s00404-023-06992-z

Purpose Although the impact of the paternal contribution to embryo quality and blastocyst formation is a well-known phenomenon, the current literature provides insufficient evidence that hyaluronan-binding sperm selection methods improve assisted reproductive treatment outcomes. Thus, we compared the cycle outcomes of morphologically selected intracytoplasmic sperm injection (ICSI) with hyaluronan binding physiological intracytoplasmic sperm injection (PICSI) cycles. Methods A total of 2415 ICSI and 400 PICSI procedures of 1630 patients who underwent in vitro fertilization cycles using a time-lapse monitoring system between 2014 and 2018 were analyzed retrospectively. Fertilization rate, embryo quality, clinical pregnancy rate, biochemical pregnancy rate and miscarriage rate were evaluated, differences in morphokinetic parameters and cycle outcomes were compared. Results In total, 85.8 and 14.2% of the whole cohort were fertilized with standard ICSI and PICSI, respectively. The proportion of fertilized oocytes did not significantly differ between groups (74.53 ± 1.33 vs. 72.92 ± 2.64, p  > 0.05). Similarly, the proportion of good-quality embryos according to the time-lapse parameters and the clinical pregnancy rate did not significantly differ between groups (71.93 ± 4.21 vs. 71.33 ± 2.64, p  > 0.05 and 45.55 ± 2.91 vs. 44.96 ± 1.25, p  > 0.05). No statistically significant differences were found between groups in clinical pregnancy rates (45.55 ± 2.91 vs. 44.96 ± 1.25, p  > 0.05). Biochemical pregnancy rates (11.24 ± 2.12 vs. 10.85 ± 1.83, p  > 0.05) and miscarriage rates (24.89 ± 3.74 vs. 27.91 ± 4.91, p  > 0.05) were not significantly different between groups. Conclusion The effects of the PICSI procedure on fertilization rate, biochemical pregnancy rate, miscarriage rate, embryo quality, and clinical pregnancy outcomes were not superior. The effect of the PICSI procedure on embryo morphokinetics was not apparent when all parameters were considered.