Adaptive laboratory evolution boosts Yarrowia lipolytica tolerance to vanillic acid

Microbial tolerance to lignocellulose-derived inhibitors, such as aromatic acids, is critical for the economical production of biofuels and biochemicals. Here, adaptive laboratory evolution was applied to improve the tolerance of Yarrowia lipolytica to a representative aromatic acid inhibitor vanill...

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Bibliographic Details
Published in:Journal of biotechnology 2023-04, Vol.367, p.42-52
Main Authors: Sha, Yuanyuan, Zhou, Linlin, Wang, Zedi, Ding, Ying, Lu, Minrui, Xu, Zhaoxian, Zhai, Rui, Jin, Mingjie
Format: Article
Language:English
Subjects:
Adaptive laboratory evolution
Lignocellulosic biorefinery
Metabolic Engineering
Transcriptome
Vanillic acid
Vanillic Acid - metabolism
Vanillic Acid - pharmacology
Yarrowia - genetics
Yarrowia - metabolism
Yarrowia lipolytica
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Summary:Microbial tolerance to lignocellulose-derived inhibitors, such as aromatic acids, is critical for the economical production of biofuels and biochemicals. Here, adaptive laboratory evolution was applied to improve the tolerance of Yarrowia lipolytica to a representative aromatic acid inhibitor vanillic acid. The transcriptome profiling of evolved strain suggested that the tolerance could be related to the up-regulation of RNA processing and multidrug transporting pathways. Further analysis by reverse engineering confirmed that the amplification of YALI0_F13475g coding for transcriptional coactivator and YALI0_E25201g coding for multidrug transporter conferred tolerance not only to vanillic acid but also towards ferulic acid, p-coumaric acid, p-hydroxybenzoic acid and syringic acid. These findings suggested that regulation of RNA processing and multidrug transporting pathways may be important for enhanced aromatic acid tolerance in Y. lipolytica. This study provides valuable genetic information for robust strain construction for lignocellulosic biorefinery. [Display omitted] •A vanillic acid tolerant Yarrowia lipolytica was obtained by adaptive evolution.•Transcriptome profile offered potential targets for robust strain construction.•Two genes contribute to increased tolerance to multiple aromatic acids.•Aromatic acid tolerance is tightly linked to transcriptional and transport processes.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2023.03.006