Integrated fermentative production and downstream processing of L-malic acid by Aspergillus wentii using cassava peel waste

[Display omitted] •L-malic acid (L-MA) production by Aspergillus wentii using cassava peel waste(CPW).•A glucose yield of 500–700 g/kg CPW was obtained by acid and enzymatic hydrolysis.•L-MA titer of 37.9 g/L and yield of 0.39 g/g was achieved on pure glucose.•Acid and enzymatic hydrolysate generate...

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Published in:Bioresource technology 2023-06, Vol.377, p.128946-128946, Article 128946
Main Authors: Gopaliya, Deeksha, Zaidi, Saniya, Srivastava, Nitin, Rani, Bhumika, Kumar, Vinod, Kumar Khare, Sunil
Format: Article
Language:English
Subjects:
Cassava peel waste (CPW)
Downstream processing
Fermentation
Glucose
Hydrolysis
L-malic acid
Manihot
Separate hydrolysis & fermentation (SHF)
Starchy waste-fermentation
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Summary:[Display omitted] •L-malic acid (L-MA) production by Aspergillus wentii using cassava peel waste(CPW).•A glucose yield of 500–700 g/kg CPW was obtained by acid and enzymatic hydrolysis.•L-MA titer of 37.9 g/L and yield of 0.39 g/g was achieved on pure glucose.•Acid and enzymatic hydrolysate generated 20.9 g/L and 33.1 g/L L-MA, respectively.•n-Butanol-based solvent extraction method resulted in 31% recovery of L-MA. L-malic acid (L-MA) is an industrially significant chemical with enormous potential. The fungal cell factories could be exploited to harvest it on large scales. In our study, Aspergillus wentii strain (MTCC 1901 T) was explored for L-MA production. Initially, the L-MA production was carried out using glucose with optimization of parameters influencing product accumulation (pH and CaCO3). The fermentation resulted in L-MA titer of 37.9 g/L with 0.39 g/g yield. Then, cassava peel waste (CPW) was used for L-MA production by separate hydrolysis and fermentation. Optimized acidic and enzymatic hydrolysis resulted in glucose release of 0.53 and 0.66 g/g CPW, respectively. The strain accumulated 20.9 g/L and 33.1 g/L L-MA with corresponding yields of 0.25 g/g and 0.34 g/g during batch cultivation using acid and enzyme hydrolysate, respectively. Finally, the produced L-MA was separated using an inexpensive solvent extraction method. Among various solvents used, n-butanol exhibited maximum L-MA extraction efficiency (31%).
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2023.128946