Structure–activity relationship analysis of activity-based probes targeting HTRA family of serine proteases

[Display omitted] High temperature requirement A serine proteases (HTRA) are ubiquitously expressed and participate in protein quality control and cellular stress responses. They are linked to several clinical illnesses, including bacterial infection, cancer, age-related macular degeneration, and ne...

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Published in:Bioorganic & medicinal chemistry letters 2023-05, Vol.87, p.129259-129259, Article 129259
Main Authors: Song, Dasom, Lee, Ji-Yu, Park, Eun-Chae, Choi, Na-Eun, Nam, Ho-Yeon, Seo, Jiwon, Lee, Jiyoun
Format: Article
Language:English
Subjects:
Activity-based probe
DegP protease
Fluorescent probe
HTRA serine protease
Peptide
Serine Endopeptidases - metabolism
Serine Proteases - metabolism
Structure-Activity Relationship
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Summary:[Display omitted] High temperature requirement A serine proteases (HTRA) are ubiquitously expressed and participate in protein quality control and cellular stress responses. They are linked to several clinical illnesses, including bacterial infection, cancer, age-related macular degeneration, and neurodegenerative diseases. In addition, several recent studies have revealed HTRAs as important biomarkers and potential therapeutic targets, necessitating the development of an effective detection method to evaluate their functional states in various disease models. We developed a new series of HTRA-targeting activity-based probes with enhanced subtype selectivity and reactivity. In conjunction with our previously developed tetrapeptide probes, we established the structure–activity relationship of the new probes for different HTRA subtypes. Our probes are cell-permeable and have potent inhibitory effects against HTRA1 and HTRA2, making them valuable for identifying and validating HTRAs as an important biomarker.
ISSN:0960-894X
1464-3405
DOI:10.1016/j.bmcl.2023.129259