Genotoxicity evaluation of orally administered styrene monomer in mice using comet, micronucleus, and Pig‐a endpoints

Male B6C3F1 mice were administered styrene monomer by oral gavage for 29 consecutive days at dose levels of 0, 75, 150, or 300 mg/kg/day. The highest dose level represented the maximum tolerated dose based on findings in a 28‐day dose range‐finding study, in which the bioavailability of orally admin...

Full description

Saved in:
Bibliographic Details
Published in:Environmental and molecular mutagenesis 2023-06, Vol.64 (5), p.282-290
Main Author: Gollapudi, B. Bhaskar
Format: Article
Language:English
Subjects:
aneugenicity
Animals
B6C3F1 mice
Bioassays
Bioavailability
Clastogenesis
clastogenicity
Comet assay
Comet Assay - methods
Deoxyribonucleic acid
DNA
DNA Damage
Duodenum
Erythrocytes
Ethyl methanesulfonate
Ethyl nitrosourea
Genotoxicity
Humans
Kidneys
Liver
Lungs
Male
Mice
Micronucleus Tests - methods
Monomers
Mutagenesis
mutation
Oral administration
Small intestine
Stomach
Styrene
Styrene - toxicity
Styrenes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Male B6C3F1 mice were administered styrene monomer by oral gavage for 29 consecutive days at dose levels of 0, 75, 150, or 300 mg/kg/day. The highest dose level represented the maximum tolerated dose based on findings in a 28‐day dose range‐finding study, in which the bioavailability of orally administered styrene was also confirmed. The positive control group received ethyl nitrosourea (ENU; 51.7 mg/kg/day) on Study Days 1–3 and ethyl methanesulfonate (EMS; 150 mg/kg/day) on Study Days 27–29 by oral gavage. Approximately 3 h following the final dose, blood was collected to assess erythrocyte Pig‐a mutant and micronucleus frequencies. DNA strand breakage was assessed in glandular stomach, duodenum, kidney, liver, and lung tissues using the alkaline comet assay. The %tail DNA for stomach, liver, lung, and kidney in the comet assay among the styrene‐treated groups was neither significantly different from the respective vehicle controls nor was there any dose‐related increasing trend in any of the tissues; results for duodenum were interpreted to be inconclusive because of technical issues. The Pig‐a and micronucleus frequencies among styrene‐treated groups also did not show significant increases relative to the vehicle controls and there was also no evidence for a dose‐related increasing trend. Thus, orally administered styrene did not induce DNA damage, mutagenesis, or clastogenesis/aneugenesis in these Organization of Economic Co‐operation and Development test guideline‐compliant genotoxicity studies. Data from these studies can contribute to the overall assessment of genotoxic hazard and risk posed to humans potentially exposed to styrene.
ISSN:0893-6692
1098-2280
DOI:10.1002/em.22540