An Optochemical Oxygen Scavenger Enabling Spatiotemporal Control of Hypoxia

We present an optochemical O2 scavenging system that enables precise spatiotemporal control of the level of hypoxia in living cells simply by adjusting the light intensity in the illuminated region. The system employs rhodamine containing a selenium or tellurium atom as an optochemical oxygen scaven...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2023-05, Vol.62 (20), p.e202217585-n/a
Main Authors: Ieda, Naoya, Sawada, Masato, Oguchi, Runa, Itoh, Masato, Hirakata, Seina, Saitoh, Daisuke, Nakao, Akito, Kawaguchi, Mitsuyasu, Sawamoto, Kazunobu, Yoshihara, Toshitada, Mori, Yasuo, Nakagawa, Hidehiko
Format: Article
Language:English
Subjects:
Calcium influx
Calcium ions
Fluorescence
Fluorescent indicators
Glutathione
HEK293 Cells
Humans
Hypoxia
Irradiation
Light intensity
Light irradiation
Luminous intensity
Oxygen
Oxygen Scavenger
Phosphorescence
Photochemical reactions
Photochemicals
Photocontrol
Radiation
Reactive Oxygen Species
Rhodamine
Scavenging
Selenium
Tellurium
TRPA1
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Summary:We present an optochemical O2 scavenging system that enables precise spatiotemporal control of the level of hypoxia in living cells simply by adjusting the light intensity in the illuminated region. The system employs rhodamine containing a selenium or tellurium atom as an optochemical oxygen scavenger that rapidly consumes O2 by photochemical reaction with glutathione as a coreductant upon visible light irradiation (560–590 nm) and has a rapid response time, within a few minutes. The glutathione‐consuming quantum yields of the system were calculated as about 5 %. The spatiotemporal O2 consuming in cultured cells was visualized with a hypoxia‐responsive fluorescence probe, MAR. Phosphorescence lifetime imaging was applied to confirmed that different light intensities could generate different levels of hypoxia. To illustrate the potential utility of this system for hypoxia research, we show that it can spatiotemporally control calcium ion (Ca2+) influx into HEK293T cells expressing the hypoxia‐responsive Ca2+ channel TRPA1. An optochemical oxygen scavenging system is reported that enables spatiotemporal control of hypoxia in cellular conditions. In this system, a selenium‐containing rhodamine derivative works as an oxygen scavenging photocatalyst with glutathione as a co‐reductant. This system consumed oxygen quickly and can establish localized hypoxic conditions even in cultured cells, providing a new chemical tool for hypoxia research.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.202217585