A HPLC-DAD method to facilitate large-scale therapeutic drug monitoring of dalbavancin

•Development of a sensitive and specific monitoring HPLC-DAD method with lower cost.•A simple extraction was performed.•The calibration curve allows the monitoring of Dalbavancin concentrations in adults and children. Dalbavancin, a long-acting lipoglycopeptide antibiotic targeting susceptible Gram-...

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Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2023-05, Vol.1222, p.123694-123694, Article 123694
Main Authors: Destere, Alexandre, Merino, Diane, Bonesso, Laurent, Lavrut, Thibaud, Bernasconni, Anaïs, Garraffo, Rodolphe, Gérard, Alexandre O., Drici, Milou-Daniel
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotic
Chromatography, High Pressure Liquid
Dalbavancin
Drug Monitoring
High performance liquid chromatography
Microbial Sensitivity Tests
Reproducibility of Results
Teicoplanin - pharmacology
Teicoplanin - therapeutic use
Therapeutic drug monitoring
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Summary:•Development of a sensitive and specific monitoring HPLC-DAD method with lower cost.•A simple extraction was performed.•The calibration curve allows the monitoring of Dalbavancin concentrations in adults and children. Dalbavancin, a long-acting lipoglycopeptide antibiotic targeting susceptible Gram-positive bacteria, is WHO critically important antibiotic, increasingly used in critical situations such as osteoarticular infections. To ensure its effectiveness and its safety, the therapeutic drug monitoring (TDM) of dalbavancin is strongly recommended. In the absence of an available minimum inhibitory concentration (MIC), the European Committee on Antimicrobial Susceptibility Testing (EUCAST) recommends a breakpoint of 0.125 mg/L for Staphylococcus aureus, corresponding to a trough target concentration of 25 mg/L. Nowadays, the TDM is usually performed using a high-performance liquid chromatography (HPLC) method coupled with a tandem mass spectrometry. However, this expensive and specialized equipment and reagents may be difficult to acquire for non-specialized laboratories. The use of HPLC coupled with diode array detector (DAD) facilitates TDM with a lower cost, while preserving the reliability of the results. Our aim was to provide a sensitive and specific method, relying on HPLC-DAD for extending the TDM of dalbavancin beyond non-specialized labs, therefore maximizing its efficiency and Benefit/risk ratio. Our method complied with the European Medicines Agency guidelines of bioanalytical validation. Irrespective of the concentrations of dalbavancin, the coefficient of variation 
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2023.123694