AFM‐TEM correlation microscopy and its application to lipid nanoparticles

So far, only a few articles have demonstrated the possibility of correlated AFM‐TEM imaging – sequential imaging of the same individual objects using atomic‐force microscopy (AFM) and transmission electron microscopy (TEM). The current work contributes to the development of this approach by giving a...

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Bibliographic Details
Published in:Microscopy research and technique 2023-07, Vol.86 (7), p.781-790
Main Authors: Bagrov, Dmitry V., Adlerberg, Vladimir V., Skryabin, Gleb O., Nikishin, Igor I., Galetsky, Sergey A., Tchevkina, Elena M., Kirpichnikov, Mikhail P., Shaitan, Konstantin V.
Format: Article
Language:English
Subjects:
Atomic force microscopy
image correlation
image registration
Imaging
lipid nanoparticles
Lipids
Liposomes
Microscopy, Atomic Force - methods
Microscopy, Electron, Transmission
Nanometrology
Nanoparticles
TEM grids
Transmission electron microscopy
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Summary:So far, only a few articles have demonstrated the possibility of correlated AFM‐TEM imaging – sequential imaging of the same individual objects using atomic‐force microscopy (AFM) and transmission electron microscopy (TEM). The current work contributes to the development of this approach by giving a step‐by‐step procedure, which yields pairs of correlated AFM‐TEM images. We describe the application of correlation AFM‐TEM microscopy to lipid nanoparticles (small extracellular vesicles and liposomes). The sizes of individual particles measured by the two methods were in good agreement, taking the tip broadening into account. The correlated AFM‐TEM imaging can be valuable for single‐particle analysis and nanometrology. AFM and TEM can be used successively to visualize the individual chosen nano‐sized objects deposited onto a TEM grid.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.24336