PHMB modified photothermally triggered nitric oxide release nanoplatform for precise synergistic therapy of wound bacterial infections

• Published in: International journal of pharmaceutics 2023-06, Vol.640, p.123014-123014, Article 123014
• Main Authors: Qi, Chenyang, Chen, Jie, Zhuang, Ying, Zhang, Yipin, Zhang, Qinqin, Tu, Jing
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Antibacterial; Bacterial Infections; Escherichia coli; Humans; Nitric Oxide; Nitric oxide release; Photothermal therapy; Prussian blue; Staphylococcus aureus; Synergistic therapy
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2023.123014

Cationic antimicrobial polymer modified NIR-controllable nitric oxide generation nanoplatform (PB-NO@PDA-PHMB) for precise synergistic therapy of wound bacterial infections. [Display omitted] Bacterial infection has been considered as a significant obstacle for wound healing. Nitric oxide (NO), as a novel alternative for antibiotics, has emerged as a promising antibacterial agent. However, the precise spatiotemporal controlled release of NO still remains a major challenge. Herein, a near-infrared (NIR) light triggered NO release nanoplatform (designated as PB-NO@PDA-PHMB) with enhanced broad-spectrum antibacterial and anti-biofilm properties was constructed. Given that PB-NO@PDA-PHMB has strong absorption in the NIR region and exhibits excellent photothermal effect, it can rapidly trigger NO release by NIR irradiation. PB-NO@PDA-PHMB can effectively contact and capture bacteria, and then exhibit synergistic effect of photothermal and gas therapy. In vitro and in vivo experiments indicated that PB-NO@PDA-PHMB exhibited excellent biocompatibility, satisfactory synergistic antibacterial efficacy and the capability of accelerating wound healing. Under NIR irradiation (808 nm, 1 W cm−2, 7 min), PB-NO@PDA-PHMB (80 μg mL−1) achieved 100% bactericidal activity against both Gram-negative bacteria Escherichia coli (E. coli) and Gram-positive bacteria Staphyloccocus aureus (S. aureus), removed 58.94% of S. aureus biofilm. Therefore, this all-in-one antibacterial nanoplatform with high NIR responsiveness provides a promising antibiotic-free strategy for bacterial infection treatment.