Antibody-free and selective detection of okadaic acid using an affinity peptide-based indirect assay

[Display omitted] •Peptide-based competitive ELISA for detection of okadaic acid was developed.•Okadaic acid-specific peptide was successfully identified and chemically synthesized.•Developed peptide-based ELISA exhibit good limit of detection and limit of quantitation.•Our developed ELISA show good...

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Bibliographic Details
Published in:Food chemistry 2023-10, Vol.422, p.136243-136243, Article 136243
Main Authors: Cho, Chae Hwan, Park, Chan Yeong, Chun, Hyang Sook, Park, Tae Jung, Park, Jong Pil
Format: Article
Language:English
Subjects:
Affinity peptide
Antibodies, Monoclonal
Humans
Marine toxin
Okadaic acid
Okadaic Acid - analysis
Peptides
Phage display
Seafood - analysis
Shellfish - analysis
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Summary:[Display omitted] •Peptide-based competitive ELISA for detection of okadaic acid was developed.•Okadaic acid-specific peptide was successfully identified and chemically synthesized.•Developed peptide-based ELISA exhibit good limit of detection and limit of quantitation.•Our developed ELISA show good performance in a complexed real shellfish samples. Okadaic acid (OA) is a type of marine biotoxin produced by some species of dinoflagellates in marine environments. Consumption of shellfish contaminated with OA can cause diarrhetic shellfish poisoning (DSP) in humans with symptoms that typically include abdominal pain, diarrhea and vomiting. In this study, we developed an affinity peptide-based direct competition enzyme-linked immunosorbent assay (dc-ELISA) for the detection of OA in real samples. The OA-specific peptide was successfully identified via M13 biopanning and a series of peptides were chemically synthesized and characterized their recognition activities. The dc-ELISA system showed good sensitivity and selectivity with a half-maximal inhibitory concentration (IC50) of 148.7 ng/mL and a limit of detection (LOD) of 5.41 ng/mL (equivalent, 21.52 ng/g). Moreover, the effectiveness of the developed dc-ELISA was validated using OA-spiked shellfish samples, and the developed dc-ELISA showed a high recovery rate. These results suggest that the affinity peptide-based dc-ELISA can be a promising tool for detecting OA in shellfish samples.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2023.136243