Benzo(ghi)perylene (BgP) a black tattoo ingredient induced skin toxicity via direct and indirect mode of DNA damage under UVA irradiation

• Published in: Chemico-biological interactions 2023-07, Vol.379, p.110508-110508, Article 110508
• Main Authors: Negi, Sandeep, Shukla, Saumya, Patel, Sunil Kumar, Vikram, Apeksha, Gaur, Prakriti, Kamar, Mohd Danish, Pathania, Diksha, Kotian, Sumana Y., Bala, Madhu, Rana, Priyanka, Bala, Lakshmi, Yadav, Akhilesh K., Ray, Ratan Singh, Dwivedi, Ashish
• Format: Article
• Language: English
• Subjects: And UVA; Benzo(ghi)perylene (BgP); DNA - metabolism; DNA Damage; Humans; Molecular docking; Molecular Docking Simulation; Skin - metabolism; Tattooing - adverse effects; UHPLC; Ultraviolet Rays - adverse effects; γ-H2AX foci
• ISSN: 0009-2797; 1872-7786
• DOI: 10.1016/j.cbi.2023.110508

Tattooing is a very common fashion trend across all the ages and gender of the society worldwide. Although skin inflammatory diseases are very frequent among tattoo users because of the active chemical ingredients used in tattoo ink, yet no ingredient-specific toxicity study has been performed. Benzo(ghi)perylene (BgP) is one of the PAHs and an important ingredient of black tattoo ink that shows strong absorption in UVA and UVB radiation of sunlight. Therefore, understanding the hazardous potential of BgP especially under UVA exposure is important for the safety of skin of tattoo users by considering the fact that penetration of UVA is in the dermis region where tattoo ingredients reside. To evaluate the hazardous potential of BgP on human skin under UVA exposure, different experimental tools i.e., in-chemico, in-silico and in-vitro were utilized. Our results illustrated that BgP photosensitized under UVA (1.5 mW/cm2) irradiation shows a degradation pattern till 4 h exposure. Photosensitized BgP reduced significant cell viability (%) at 1 μg/ml concentration. However, the pretreatment of singlet and hydroxyl radical quenchers, restoration of cell viability observed, confirmed the role of type-I and type-II photodynamic reactions in phototoxicity of BgP. Further, intracellular uptake of BgP in HaCaT cells was estimated and confirmed by UHPLC analysis. Molecular docking of BgP with DNA and formation of γ-H2AX foci demonstrated the DNA intercalation and double-stranded DNA damaging potential of BgP. Furthermore, acridine orange and ethidium bromide (AO/EB) dual staining showed apoptotic cell death via photosensitized BgP under UVA irradiation. The above findings suggest that BgP reached the human skin cell and induced dermal toxicity via direct and indirect mode of DNA damage under UVA exposure finally promoting the skin cell death. Thus, BgP-containing tattoo ink may be hazardous and may induce skin damage and diseases, especially in presence of UVA radiation of sunlight. To minimize the risk of skin diseases from synthetic ingredients in tattoo ink, the study highlights the importance of developing eco-friendly and skin-friendly tattoo ingredients by companies. [Display omitted] •Benzo(ghi)perylene (BgP) a PAHs and ingredient of black tattoo ink.•BgP photosensitized under UVA (1.5 mW/cm2) shows degradation.•Role of type-I and type-II photodynamic reactions in phototoxicity.•γ-H2AX foci and DNA intercalation shows DNA damaging potential.•AO/EB dual staining