The concordance of length- and sequence-based STRs used in forensic markers with guidance for practice

Next-generation sequencing (NGS) technology has shed light on every aspect of genetic discoveries, including forensic genetics. The Miseq® FGx Forensic Genetic System (Verogen) is one of the pioneering forensic NGS that provided a complete system from library preparation to data analysis. The system...

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Bibliographic Details
Published in:Forensic science international 2023-07, Vol.348, p.111729-111729, Article 111729
Main Authors: Sathirapatya, Tikumphorn, Worrapitirungsi, Wikanda, Sukawutthiya, Poonyapat, Noh, Hasnee, Pitiwararom, Rachtipan, Vongpaisarnsin, Kornkiat
Format: Article
Language:English
Subjects:
Data analysis
DNA Fingerprinting - methods
Forensic
Forensic Genetics - methods
Forensic science
Forensic sciences
Genetics
Genotype & phenotype
High-Throughput Nucleotide Sequencing - methods
Humans
Microsatellite Repeats
Next generation sequencing
Nomenclature
Population data
Population studies
Sequence Analysis, DNA
Short tandem repeat
Short tandem repeats
Software
Thai
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Summary:Next-generation sequencing (NGS) technology has shed light on every aspect of genetic discoveries, including forensic genetics. The Miseq® FGx Forensic Genetic System (Verogen) is one of the pioneering forensic NGS that provided a complete system from library preparation to data analysis. The system has been validated by several studies and led to a more practical aspect. Short tandem repeat (STR) is a well-established marker that was designed specifically for human individualization. Since NGS provides different data from fragment analysis, a new STR nomenclature is established to make NGS backward compatible with the previous data. In this study, Thai population were used to evaluate the Miseq® FGx Forensic genetic system (Verogen) in practical aspect, including concordance study and forensic population parameters. In summary, we purposed a practical guideline for sequence-based STRs. •Concordance study of allele calling between CE length-based, UAS and STRinNGS v2.0.•Allele frequencies and population forensic parameters of aSTRs and Y-STRs were assessed from 210 Thai population.•Some variations and repeat structures in STRs are outstanding in Asian population.•Practical guidelines for sequence-based STRs was purposed for NGS implementation in forensic field.
ISSN:0379-0738
1872-6283
DOI:10.1016/j.forsciint.2023.111729