Integrated genome‐wide association study and QTL mapping reveals qSa‐3A associated with English grain aphid, Sitobion avenae (Fabricius) resistance in wheat

• Published in: Pest management science 2023-10, Vol.79 (10), p.3970-3978
• Main Authors: Zhao, Yue, Yan, Xuefang, Zeng, Zhankui, Zhao, Dehui, Chen, Peng, Wang, Yuying, Chen, Feng, Wang, Chunping
• Format: Article
• Language: English
• Subjects: Antibiosis; Chromosomes; Cloning; Firing rate; Gene mapping; Genetic improvement; Genomes; Grain; GWAS; Inbreeding; Loci; Nucleotides; Pest resistance; Plant breeding; Polymorphism; QTL mapping; Quantitative trait loci; Single-nucleotide polymorphism; Sitobion avenae; Sitobion avenae (Fabricius); three classic resistance mechanisms; Triticum aestivum L; Wheat
• ISSN: 1526-498X; 1526-4998
• DOI: 10.1002/ps.7598

BACKGROUND The English grain aphid, Sitobion avenae (Fabricius), is a devastating pest impacts yield and quality in wheat (Triticum aestivum L.). Breeding resistant wheat varieties and detecting resistance genes are important strategies to control aphid. RESULTS In this study, we evaluated the number of aphids per spike, the rate of thousand kernel weight decrease and aphid index based on three classic resistance mechanisms (antibiosis, tolerance and antixenosis), and detected SNPs/QTLs for resistance to S. avenae in a natural population of 163 varieties with 20 689 high‐quality single‐nucleotide polymorphism (SNP) markers and recombinant inbred line (RIL) population of 164 lines with 3627 diversity arrays technology (DArT) markers. Results showed that 83 loci significantly associated with S. avenae antibiosis and 182 loci significantly associated with S. avenae tolerance were detected by genome‐wide association study (GWAS), explaining 6.47–15.82% and 8.36–35.61% of the phenotypic variances, respectively. The wsnp_Ku_c4568_8243646 detected in two periods was localized at 34.52 Mb on chromosome 3AS. Then, we confirmed a stable QSa.haust‐3A.2 explained 11.19–20.10% of the phenotypic variances in two periods with S. avenae antixenosis in the physical interval of 37.49–37.50 Mb on chromosome 3A in the RIL population. Therefore, a narrow region in the physical interval of 34.52–37.50 Mb on chromosome 3AS was named as qSa‐3A, which was a new locus between wsnp_Ku_c4568_8243646 and QSa.haust‐3A.2 associated with S. avenae resistance. CONCLUSION We found qSa‐3A was a new locus associated with S. avenae resistance. The results could be applied in gene cloning and genetic improvement of S. avenae resistance in wheat. © 2023 Society of Chemical Industry. The key finding: The qSa‐3A associated with S. avenae resistance was detected in the physical interval of 34.52–37.50 Mb on chromosome 3AS in wheat by combining GWAS and QTL mapping.