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Light-activated controlled release of camptothecin by engineering porous materials: the ship in a bottle concept in drug delivery
Many systems for controlled drug release have been developed using different types of nanoparticles modified with azobenzene moieties. In these systems, drug release is often triggered by UV irradiation (either direct or using a near-infrared photosensitizer). These drug delivery systems often face...
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Published in: | Nanoscale 2023-08, Vol.15 (30), p.12506-12517 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Many systems for controlled drug release have been developed using different types of nanoparticles modified with azobenzene moieties. In these systems, drug release is often triggered by UV irradiation (either direct or using a near-infrared photosensitizer). These drug delivery systems often face challenges to their use, such as their lack of stability in physiological environments and concerns about their toxicity and bioavailability, that have hindered their translation from pre-clinical studies to clinical trials. Here, we propose a conceptual change by shifting photoswitching activity from the vehicle (nanoparticle) to the load (drug). In this "ship in a bottle" concept, the molecule to be delivered is trapped within a porous nanoparticle and its release is accomplished through a photoisomerization process. Using molecular dynamics, we designed and synthesized a photoswitchable prodrug of the antitumor drug camptothecin that contains an azobenzene functionality, and we have prepared porous silica nanoparticles with pore diameters designed to limit its release when in the
form. Molecular modelling was used to show that the
isomer was smaller and better able to pass through the pores than the
isomer, which was confirmed by stochastic optical reconstruction microscopy (STORM). Thus, prodrug-loaded nanoparticles were prepared by loading the
prodrug and then using UV irradiation to convert
to
isomers, trapping them, within the pores. Release of the prodrug was then accomplished by using a different UV wavelength to convert
isomers back to
. In this way, prodrug encapsulation and release could be achieved "on demand" through controlled
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photoisomerization, which allowed the prodrug to be delivered safely and its release to be triggered precisely at the region of interest. Finally, the intracellular release and cytotoxic activity of this novel drug delivery system has been validated in several human cell lines, confirming the ability of this system to accurately control the release of the camptothecin prodrug. |
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ISSN: | 2040-3364 2040-3372 |
DOI: | 10.1039/d3nr00642e |