Mutual influence of cholesterol esterase and pseudocholinesterase on the biodegradation of dental composites

• Published in: Biomaterials 2004-05, Vol.25 (10), p.1787-1793
• Main Authors: Finer, Y, Jaffer, F, Santerre, J.P
• Format: Article
• Language: English
• Subjects: Acrylic Resins - chemistry; Biodegradation, Environmental; Bisphenol A-Glycidyl Methacrylate - chemistry; Butyrylcholinesterase - chemistry; Composite Resins - chemistry; Degradation; Dental materials; Dental Materials - chemistry; Drug Synergism; Enzyme Activation; Enzyme Stability; Esterases; Humans; Hydrolysis; Methyl methacrylates; Polyethylene Glycols - chemistry; Polymethacrylic Acids - chemistry; Polyurethanes - chemistry; Resins; Saliva - chemistry; Sterol Esterase - chemistry
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2003.08.029

It has been demonstrated that human saliva contains cholesterol esterase (CE)- and pseudocholinesterase (PCE)-like hydrolase activities. While PCE has been shown to preferentially degrade triethylene glycol dimethacrylate (TEGDMA) and its derivatives, CE has a greater catalytic effect on the breakdown of bis-phenol-A-diglycidyl dimethacrylate (bisGMA) components in composite dental resins. The current study seeks to determine if there is a mutual influence between the different esterases with respect to the biodegradation of resin composite. Photopolymerized model composite resin samples (containing 60% by weight fraction of silanated barium glass filler) based on bisGMA/TEGDMA (bis) or urethane-modified bisGMA/TEGDMA/bisEMA (ubis) monomers were incubated in buffer, CE and/or PCE solutions (pH=7.0, 37°C) for 8 and 16 days. The incubation solutions were analyzed for degradation products using high-performance liquid chromatography, UV spectroscopy and mass spectrometry. In the bis system, higher amounts ( p