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Mutual influence of cholesterol esterase and pseudocholinesterase on the biodegradation of dental composites
It has been demonstrated that human saliva contains cholesterol esterase (CE)- and pseudocholinesterase (PCE)-like hydrolase activities. While PCE has been shown to preferentially degrade triethylene glycol dimethacrylate (TEGDMA) and its derivatives, CE has a greater catalytic effect on the breakdo...
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| Published in: | Biomaterials 2004-05, Vol.25 (10), p.1787-1793 |
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| creator | Finer, Y Jaffer, F Santerre, J.P |
| description | It has been demonstrated that human saliva contains cholesterol esterase (CE)- and pseudocholinesterase (PCE)-like hydrolase activities. While PCE has been shown to preferentially degrade triethylene glycol dimethacrylate (TEGDMA) and its derivatives, CE has a greater catalytic effect on the breakdown of bis-phenol-A-diglycidyl dimethacrylate (bisGMA) components in composite dental resins. The current study seeks to determine if there is a mutual influence between the different esterases with respect to the biodegradation of resin composite. Photopolymerized model composite resin samples (containing 60% by weight fraction of silanated barium glass filler) based on bisGMA/TEGDMA (bis) or urethane-modified bisGMA/TEGDMA/bisEMA (ubis) monomers were incubated in buffer, CE and/or PCE solutions (pH=7.0, 37°C) for 8 and 16 days. The incubation solutions were analyzed for degradation products using high-performance liquid chromatography, UV spectroscopy and mass spectrometry. In the bis system, higher amounts (
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| doi_str_mv | 10.1016/j.biomaterials.2003.08.029 |
| format | article |
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p<0.05) of a bisGMA derived product, bishydroxy-propoxyphenyl-propane (bisHPPP), were detected in the combined enzyme group as compared to the sum of the two individual enzyme groups. In the ubis system, similar comparisons showed that higher levels (
p<0.05) of bisHPPP were detected in the combined group at 8 days while higher amounts (
p<0.05) of a bisEMA derived product, ethoxylated bis-phenol A, were detected in the combined group at 16 days. The study concluded that CE and PCE act synergistically to increase the biodegradation of both composite resin materials.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2003.08.029</identifier><identifier>PMID: 14738842</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Acrylic Resins - chemistry ; Biodegradation, Environmental ; Bisphenol A-Glycidyl Methacrylate - chemistry ; Butyrylcholinesterase - chemistry ; Composite Resins - chemistry ; Degradation ; Dental materials ; Dental Materials - chemistry ; Drug Synergism ; Enzyme Activation ; Enzyme Stability ; Esterases ; Humans ; Hydrolysis ; Methyl methacrylates ; Polyethylene Glycols - chemistry ; Polymethacrylic Acids - chemistry ; Polyurethanes - chemistry ; Resins ; Saliva - chemistry ; Sterol Esterase - chemistry</subject><ispartof>Biomaterials, 2004-05, Vol.25 (10), p.1787-1793</ispartof><rights>2003 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-955dfbdbd38a295b0e3bdd4abdec534d36acd7f9e5f553c954f332421a639ba53</citedby><cites>FETCH-LOGICAL-c438t-955dfbdbd38a295b0e3bdd4abdec534d36acd7f9e5f553c954f332421a639ba53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14738842$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Finer, Y</creatorcontrib><creatorcontrib>Jaffer, F</creatorcontrib><creatorcontrib>Santerre, J.P</creatorcontrib><title>Mutual influence of cholesterol esterase and pseudocholinesterase on the biodegradation of dental composites</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>It has been demonstrated that human saliva contains cholesterol esterase (CE)- and pseudocholinesterase (PCE)-like hydrolase activities. While PCE has been shown to preferentially degrade triethylene glycol dimethacrylate (TEGDMA) and its derivatives, CE has a greater catalytic effect on the breakdown of bis-phenol-A-diglycidyl dimethacrylate (bisGMA) components in composite dental resins. The current study seeks to determine if there is a mutual influence between the different esterases with respect to the biodegradation of resin composite. Photopolymerized model composite resin samples (containing 60% by weight fraction of silanated barium glass filler) based on bisGMA/TEGDMA (bis) or urethane-modified bisGMA/TEGDMA/bisEMA (ubis) monomers were incubated in buffer, CE and/or PCE solutions (pH=7.0, 37°C) for 8 and 16 days. The incubation solutions were analyzed for degradation products using high-performance liquid chromatography, UV spectroscopy and mass spectrometry. In the bis system, higher amounts (
p<0.05) of a bisGMA derived product, bishydroxy-propoxyphenyl-propane (bisHPPP), were detected in the combined enzyme group as compared to the sum of the two individual enzyme groups. In the ubis system, similar comparisons showed that higher levels (
p<0.05) of bisHPPP were detected in the combined group at 8 days while higher amounts (
p<0.05) of a bisEMA derived product, ethoxylated bis-phenol A, were detected in the combined group at 16 days. The study concluded that CE and PCE act synergistically to increase the biodegradation of both composite resin materials.</description><subject>Acrylic Resins - chemistry</subject><subject>Biodegradation, Environmental</subject><subject>Bisphenol A-Glycidyl Methacrylate - chemistry</subject><subject>Butyrylcholinesterase - chemistry</subject><subject>Composite Resins - chemistry</subject><subject>Degradation</subject><subject>Dental materials</subject><subject>Dental Materials - chemistry</subject><subject>Drug Synergism</subject><subject>Enzyme Activation</subject><subject>Enzyme Stability</subject><subject>Esterases</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>Methyl methacrylates</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Polymethacrylic Acids - chemistry</subject><subject>Polyurethanes - chemistry</subject><subject>Resins</subject><subject>Saliva - chemistry</subject><subject>Sterol Esterase - chemistry</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqNkctu2zAQRYkgRew4_YVC6CI7qXxaZHaF8wRcdNOsCYoc1TQk0SGlAv37ULWRdNeuBuTcuXMxB6HPBFcEk_WXfdX40JsRojddqijGrMKywlSdoSWRtSyFwuIcLTHhtFRrQhfoMqU9zm_M6QVaEF4zKTldou7bNE6mK_zQdhMMForQFnYXOkjZP3TFn2oSFGZwxSHB5MLc9sNbIwzFuIMiZ3LwMxpnRp-_so2DYczWNvSHkPwI6Qp9aHNi-HiqK_R8f_dj81huvz88bb5uS8uZHEslhGsb1zgmDVWiwcAa57hpHFjBuGNrY13dKhCtEMwqwVvGKKfErJlqjGArdH30PcTwMuWguvfJQteZAcKUNJWUc8LIP4WklqRWpM7Cm6PQxpBShFYfou9N_K0J1jMUvdd_Q9EzFI2lzlDy8KfTlqnpwb2Pnihkwe1RAPkovzxEnayfYTgfwY7aBf8_e14B8ASoVg</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>Finer, Y</creator><creator>Jaffer, F</creator><creator>Santerre, J.P</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>F28</scope><scope>JG9</scope></search><sort><creationdate>20040501</creationdate><title>Mutual influence of cholesterol esterase and pseudocholinesterase on the biodegradation of dental composites</title><author>Finer, Y ; Jaffer, F ; Santerre, J.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-955dfbdbd38a295b0e3bdd4abdec534d36acd7f9e5f553c954f332421a639ba53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Acrylic Resins - chemistry</topic><topic>Biodegradation, Environmental</topic><topic>Bisphenol A-Glycidyl Methacrylate - chemistry</topic><topic>Butyrylcholinesterase - chemistry</topic><topic>Composite Resins - chemistry</topic><topic>Degradation</topic><topic>Dental materials</topic><topic>Dental Materials - chemistry</topic><topic>Drug Synergism</topic><topic>Enzyme Activation</topic><topic>Enzyme Stability</topic><topic>Esterases</topic><topic>Humans</topic><topic>Hydrolysis</topic><topic>Methyl methacrylates</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Polymethacrylic Acids - chemistry</topic><topic>Polyurethanes - chemistry</topic><topic>Resins</topic><topic>Saliva - chemistry</topic><topic>Sterol Esterase - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Finer, Y</creatorcontrib><creatorcontrib>Jaffer, F</creatorcontrib><creatorcontrib>Santerre, J.P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Finer, Y</au><au>Jaffer, F</au><au>Santerre, J.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mutual influence of cholesterol esterase and pseudocholinesterase on the biodegradation of dental composites</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>25</volume><issue>10</issue><spage>1787</spage><epage>1793</epage><pages>1787-1793</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>It has been demonstrated that human saliva contains cholesterol esterase (CE)- and pseudocholinesterase (PCE)-like hydrolase activities. While PCE has been shown to preferentially degrade triethylene glycol dimethacrylate (TEGDMA) and its derivatives, CE has a greater catalytic effect on the breakdown of bis-phenol-A-diglycidyl dimethacrylate (bisGMA) components in composite dental resins. The current study seeks to determine if there is a mutual influence between the different esterases with respect to the biodegradation of resin composite. Photopolymerized model composite resin samples (containing 60% by weight fraction of silanated barium glass filler) based on bisGMA/TEGDMA (bis) or urethane-modified bisGMA/TEGDMA/bisEMA (ubis) monomers were incubated in buffer, CE and/or PCE solutions (pH=7.0, 37°C) for 8 and 16 days. The incubation solutions were analyzed for degradation products using high-performance liquid chromatography, UV spectroscopy and mass spectrometry. In the bis system, higher amounts (
p<0.05) of a bisGMA derived product, bishydroxy-propoxyphenyl-propane (bisHPPP), were detected in the combined enzyme group as compared to the sum of the two individual enzyme groups. In the ubis system, similar comparisons showed that higher levels (
p<0.05) of bisHPPP were detected in the combined group at 8 days while higher amounts (
p<0.05) of a bisEMA derived product, ethoxylated bis-phenol A, were detected in the combined group at 16 days. The study concluded that CE and PCE act synergistically to increase the biodegradation of both composite resin materials.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>14738842</pmid><doi>10.1016/j.biomaterials.2003.08.029</doi><tpages>7</tpages></addata></record> |
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| subjects | Acrylic Resins - chemistry Biodegradation, Environmental Bisphenol A-Glycidyl Methacrylate - chemistry Butyrylcholinesterase - chemistry Composite Resins - chemistry Degradation Dental materials Dental Materials - chemistry Drug Synergism Enzyme Activation Enzyme Stability Esterases Humans Hydrolysis Methyl methacrylates Polyethylene Glycols - chemistry Polymethacrylic Acids - chemistry Polyurethanes - chemistry Resins Saliva - chemistry Sterol Esterase - chemistry |
| title | Mutual influence of cholesterol esterase and pseudocholinesterase on the biodegradation of dental composites |
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