Fenofibrate attenuates asthma features in an ovalbumin-induced mouse model via suppressing NF-κB binding activity

• Published in: Respiratory physiology & neurobiology 2023-08, Vol.314, p.104083-104083, Article 104083
• Main Authors: Alhirmizi, Ibraheem Akram Omar, Uysal, Fatma, Arslan, Seyfullah Oktay, Özünlü, Saliha Ayşenur Çam, Koç, Ayşegül, Parlar, Ali, Bayram, Keziban Korkmaz
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Asthma - chemically induced; Asthma - drug therapy; Asthma - metabolism; Bronchoalveolar Lavage Fluid; Cytokines - metabolism; Disease Models, Animal; Fenofibrate; Fenofibrate - metabolism; Fenofibrate - pharmacology; Fenofibrate - therapeutic use; Hypersensitivity - drug therapy; Immunoglobulin E - metabolism; Immunoglobulin E - pharmacology; Interleukin-13 - metabolism; Interleukin-5 - metabolism; Lung - metabolism; Mice; Mice, Inbred BALB C; NF-kappa B - metabolism; NF-κB-binding activity; Ovalbumin - pharmacology; Ovalbumin-induced asthma; Whole-body Plethysmography
• ISSN: 1569-9048; 1878-1519
• DOI: 10.1016/j.resp.2023.104083

Asthma is a chronic inflammatory disease of the airways with a high prevalence. Asthma has a complex pathophysiology and about 5–10% of patients are not fully responsive to the currently available treatments. The aim of this study is to investigate the involvement of NF-κB in the effects of fenofibrate on a mouse model of allergic asthma. A total of 49 BALB/c mice were randomly distributed into 7 groups (n = 7). Allergic asthma model was created by administering i.p. injections of ovalbumin on days 0, 14 and 21, followed by provocation with inhaled ovalbumin on days 28, 29 and 30. Fenofibrate was orally given in 3 different doses; 1, 10 and 30 mg/kg through days 21–30 of the experiment. On day 31, pulmonary function test using whole body plethysmography was performed. The mice were sacrificed 24 h later. Blood samples were obtained, and serum of each sample was separated for IgE determination. Bronchoalveolar lavage fluid (BALF) and lung tissues were collected to measure IL-5 and IL-13 levels. Nuclear extracts of lung tissues were employed to assess nuclear factor kappa B (NF-κB) p65 binding activity. Enhanced Pause (Penh) values were significantly increased in ovalbumin-sensitized and challenged mice (p