Decellularized Vascular Scaffolds Derived from Bovine Placenta Blood Vessels

Diseases associated with the circulatory system are the main causes of worldwide morbidity and mortality, implying the need for vascular implants. Thus, the production of vascular biomaterials has proven to be a promising alternative to therapies used in studies and research related to vascular phys...

Full description

Saved in:
Bibliographic Details
Published in:Arquivos brasileiros de cardiologia 2023, Vol.120 (6), p.e20220816-e20220816
Main Authors: Oliveira, Tarley Santos, Smirnow, Igor, Santee, Kadija Mohamed, Miglino, Maria Angelica, Barreto, Rodrigo da Silva Nunes
Format: Article
Language:English
Subjects:
Animals
Biocompatible Materials
Cardiovascular Agents
Cattle
Endothelial Cells
Female
Humans
Placenta
Pregnancy
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Diseases associated with the circulatory system are the main causes of worldwide morbidity and mortality, implying the need for vascular implants. Thus, the production of vascular biomaterials has proven to be a promising alternative to therapies used in studies and research related to vascular physiology. The present project aims to achieve the artificial development of blood vessels through the recellularization of vascular scaffolds derived from bovine placental vessels. The chorioallantoic surface of the bovine placenta was used to produce decellularized biomaterials. For recellularization, 2.5 x 104 endothelial cells were seeded above each decellularized vessel fragment during three or seven days, when culture were interrupted, and the fragments were fixed for cell attachment analysis. Decellularized and recellularized biomaterials were evaluated by basic histology, scanning electron microscopy, and immunohistochemistry. The decellularization process produced vessels that maintained natural structure and elastin content, and no cells or gDNA remains were observed. Endothelial precursor cells were also attached to lumen and external surface of the decellularized vessel.Conclusion: Our results show a possibility of future uses of this biomaterial in cardiovascular medicine, as in the development of engineered vessels.
ISSN:1678-4170
DOI:10.36660/abc.20220816