Study on the structure, antioxidant activity and degradation pattern of polysaccharides isolated from lotus seedpod

• Published in: Carbohydrate polymers 2023-09, Vol.316, p.121065-121065, Article 121065
• Main Authors: Yuan, Shuwei, Wang, Jiahui, Li, Xiang, Zhu, Xun, Zhang, Zhenqing, Li, Duxin
• Format: Article
• Language: English
• Subjects: Bioactivity; Degradation pattern; Lotus Seedpod; Polysaccharide; Structure elucidation; Structure-activity relationship
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2023.121065

The lotus (Nelumbo nucifera Gaertn.) is the largest aquatic vegetable in Asia. The lotus seedpod (LS) is an inedible part of the mature flower receptacle of the lotus plant. However, the polysaccharide isolated from the receptacle has been less studied. The purification of LS resulted in two polysaccharides (LSP-1 and LSP-2). Both polysaccharides were found to be medium-sized HG pectin, with a Mw of 74 kDa. Their structures were elucidated via GC–MS and NMR spectrum and proposed as the repeating sugar units of GalA connected via α-1,4-glycosidic linkage, with LSP-1 having a higher degree of esterification. They have certain content of antioxidant and immunomodulatory activities. The esterification of HG pectin would have an adverse effect on these activities. Furthermore, the degradation pattern and kinetics of LSPs by pectinase conformed to the Michaelis-Menten model. There is a large amount of LS, resulting from the by-product of locus seed production, and thus a promising source for the isolation of the polysaccharide. The findings of the structure, bioactivities, and degradation property provide the chemical basis for their applications in the food and pharmaceutical industries. [Display omitted]