Metabolic response induced by abiotic elicitors in Anacardium othonianum seedlings

• Published in: Natural product research 2024-06, Vol.38 (11), p.1938-1944
• Main Authors: Nascimento Pedroso, Rita Cássia, Gazolla, Ana Paula, Hara, Alessandra Cristina Boffino de Almeida Monteiro, Sales, Juliana de Fátima, Silva, Fabiano Guimarães, Dias, Herbert Junior, Crotti, Antônio Eduardo Miller, Andrade Silva, Márcio Luís, Cunha, Wilson Roberto, Pauletti, Patrícia Mendonça, Jesus, Ester Gonçalves de, Santos, Mario F. C., Januário, Ana Helena
• Format: Article
• Language: English
• Subjects: Abiotic factors; Acetates; Acetic acid; alpha-Tocopherol; Anacardiaceae; Anacardium - chemistry; Anacardium othonianum; cashew tree; Chemical composition; Composition; Ethyl acetate; Gas Chromatography-Mass Spectrometry; GC/MS; Light quality; Metabolic response; Metabolites; Plant culture; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Plant Leaves - metabolism; Salicylic acid; Salicylic Acid - pharmacology; Seedlings; Seedlings - drug effects; Seedlings - metabolism; Silver; Silver nitrate; Silver Nitrate - pharmacology; Tocopherol; Volatile compounds; Volatile Organic Compounds - analysis; Volatile Organic Compounds - metabolism; Volatile Organic Compounds - pharmacology
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2023.2225122

Applying elicitors in plants growing under natural conditions change the metabolic content of volatile compounds extracted from their tissues. Here, we have investigated how abiotic elicitors influence the volatile composition of the ethyl acetate extract of Anacardium othonianum seedlings propagated in vitro and ex-vitro cultures. Abiotic factors such as salicylic acid, silver nitrate, light quality, and cultivation mode affected the composition of volatile compounds in ethyl acetate extract of A. othonianum leaves seedlings. GC/MS analysis demonstrated that α-tocopherol was the main metabolite in cultures added with chemical elicitors, reaching 22.48 ± 4.06% in the case of 30 µM salicylic acid. Higher concentrations of salicylic acid decreased the amount of α-tocopherol. The addition of AgNO 3 had a linear effect on α-tocopherol. Thus, the production of this metabolite indicates that salicylic acid and AgNO 3 elicitors positively affect α-tocopherol production at specific concentrations helping to understand how plant culture can be better manipulated.