Genome-wide analysis of the GDSL esterase/lipase family genes in Physcomitrium patens and the involvement of GELP31 in spore germination

• Published in: Molecular genetics and genomics : MGG 2023-09, Vol.298 (5), p.1155-1172
• Main Authors: Barker, Elizabeth I., Rabbi, Fazle, Brisbourne, Wyllie A., Aparato, Vincent P. M., Escarrega Valenzuela, Vania, Renzaglia, Karen S., Suh, Dae-Yeon
• Format: Article
• Language: English
• Subjects: Animal Genetics and Genomics; Biochemistry; Biomedical and Life Sciences; Cutin; Dehydration; Esterase; Genes; Genomes; Human Genetics; Hydrophobicity; Life Sciences; Lipase; Lipid metabolism; Metabolism; Microbial Genetics and Genomics; Original Article; Pathogens; Phylogenetics; Phylogeny; Physcomitrium patens; Plant Genetics and Genomics; Plants; Proteins; Pseudogenes; Spore germination; Sporophytes
• ISSN: 1617-4615; 1617-4623
• DOI: 10.1007/s00438-023-02041-1

In plants, the ability to produce hydrophobic substances that would provide protection from dehydration was required for the transition to land. This genome-wide investigation outlines the evolution of GDSL-type esterase/lipase (GELP) proteins in the moss Physcomitrium patens and suggests possible functions of some genes. GELP proteins play roles in the formation of hydrophobic polymers such as cutin and suberin that protect against dehydration and pathogen attack. GELP proteins are also implicated in processes such as pollen development and seed metabolism and germination. The P. patens GELP gene family comprises 48 genes and 14 pseudogenes. Phylogenetic analysis of all P. patens GELP sequences along with vascular plant GELP proteins with reported functions revealed that the P. patens genes clustered within previously identified A, B and C clades. A duplication model predicting the expansion of the GELP gene family within the P. patens lineage was constructed. Expression analysis combined with phylogenetic analysis suggested candidate genes for functions such as defence against pathogens, cutin metabolism, spore development and spore germination. The presence of relatively fewer GELP genes in P. patens may reduce the occurrence of functional redundancy that complicates the characterization of vascular plant GELP genes. Knockout lines of GELP31 , which is highly expressed in sporophytes, were constructed. Gelp31 spores contained amorphous oil bodies and germinated late, suggesting (a) role(s) of GELP31 in lipid metabolism in spore development or germination. Future knockout studies of other candidate GELP genes will further elucidate the relationship between expansion of the family and the ability to withstand the harsh land environment.