MnO2 nanosheets and gold nanoparticles supported electrochemical detection of circulating tumor cells

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2023-09, Vol.229, p.113482-113482, Article 113482
• Main Authors: Yu, Nong, Ma, Guifeng, Chen, Yuyao, Huang, Shan, Gong, Yalei, Li, Shuangshuang, Gu, Haiqin, You, Honglan, Miao, Peng
• Format: Article
• Language: English
• Subjects: Aptasensor; Circulating tumor cells; Electrochemistry; Gold nanoparticles; Manganese dioxide nanosheets
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2023.113482

The concentration of circulating tumor cells (CTCs) in peripheral blood is strongly correlated with the progress of certain metastatic cancers. In this study, we have developed a novel and facile electrochemical biosensor for the detection of CTCs based on the use of manganese dioxide nanosheets (MnO2 NSs) and gold nanoparticles (AuNPs). Aptamer sequence of target cell is modified on the surface of AuNPs for specifical recognition. With low-speed centrifugation, numerous AuNPs@DNA can be removed from the supernatant. On the other hand, MnO2 NSs are modified on the electrode surface to capture unreacted AuNPs@DNA. The declined electrochemical signal intensity can be used to reflect the level of CTCs. This biosensor achieves a wide linear range from 10 to 104 cells mL−1 and a limit of detection as low as 3 cells mL−1. Due to the specific aptamer as the recognition element, interfering cells can be successfully distinguished and this method performs satisfactorily in clinical samples. Therefore, it has great potential to be used as a powerful tool benefiting rare cells analysis and the investigation of dynamics of cellular interactions. [Display omitted] •A highly sensitive electrochemical cytosensor for the detection of circulating tumor cells is developed.•Aptamer sequence is modified on the surface of gold nanoparticles for specific recognition.•Low speed centrifugation is performed to remove signal labeled gold nanoparticles in the supernatant.•Screen-printed electrode modified with MnO2 nanosheets is utilized to load gold nanoparticles for signal readout.