A novel trans-acting lncRNA of ACTG1 that induces the remodeling of ovarian follicles

Previous studies have implicated the attractive role of long noncoding RNAs (lncRNAs) in the remodeling of mammalian tissues. The migration of granulosa cells (GCs), which are the main supporting cells in ovarian follicles, stimulates the follicular remodeling. Here, with the cultured GCs as the fol...

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Published in:International journal of biological macromolecules 2023-07, Vol.242 (Pt 4), p.125170-125170, Article 125170
Main Authors: Li, Nian, Zhou, Yinqi, Cai, Jiali, Wang, Yifei, Zhou, Xiaofeng, Hu, Mengting, Li, Yubin, Zhang, Hao, Li, Jiaqi, Cai, Bing, Yuan, Xiaolong
Format: Article
Language:English
Subjects:
acetylation
actin
apoptosis
Follicular remodeling
Granulosa cells
histones
lysine
mammals
Trans-acting lncRNAs
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Summary:Previous studies have implicated the attractive role of long noncoding RNAs (lncRNAs) in the remodeling of mammalian tissues. The migration of granulosa cells (GCs), which are the main supporting cells in ovarian follicles, stimulates the follicular remodeling. Here, with the cultured GCs as the follicular model, the actin gamma 1 (ACTG1) was observed to significantly promote the migration and proliferation while inhibit the apoptosis of GCs, suggesting that ACTG1 was required for ovarian remodeling. Moreover, we identified the trans-regulatory lncRNA of ACTG1 (TRLA), which was epigenetically targeted by histone H3 lysine 4 acetylation (H3K4ac). Mechanistically, the 2–375 nt of TRLA bound to ACTG1's mRNA to increase the expression of ACTG1. Furthermore, TRLA facilitated the migration and proliferation while inhibited the apoptosis of GCs, thereby accelerating follicular remodeling. Besides, TRLA acted as a ceRNA for miR-26a to increase the expression of high-mobility group AT-hook 1 (HMGA1). Collectively, TRLA induces the remodeling of ovarian follicles via complementary to ACTG1's mRNA and regulating miR-26a/HMGA1 axis in GCs. These observations revealed a novel and promising trans-acting lncRNA mechanism mediated by H3K4ac, and TRLA might be a new target to restore follicular remodeling and development.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2023.125170