Effect of gamma and Ultraviolet-C sterilization on BMP-7 level of indigenously prepared demineralized freeze-dried bone allograft

The presence of bone morphogenetic proteins in demineralized freeze-dried bone allograft (DFDBA) are responsible for developing hard tissues in intraosseous defects. The most common mode of sterilization of bone allografts, i.e., Gamma rays, have dramatic effects on the structural and biological pro...

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Published in:Cell and tissue banking 2024-06, Vol.25 (2), p.475-484
Main Authors: Kamal, Zainab, Lamba, Arundeep Kaur, Faraz, Farrukh, Tandon, Shruti, Datta, Archita, Ansari, Nasreen, Madni, Zaid Kamal, Pandey, Jaya
Format: Article
Language:English
Subjects:
Allografts
Allografts - radiation effects
Animals
Biomedical and Life Sciences
Biomedicine
Bone grafts
Bone morphogenetic protein 7
Bone Morphogenetic Protein 7 - radiation effects
Bone Transplantation
Cell Biology
Freeze Drying
Full Length Paper
Gamma Rays
Humans
Life Sciences
Radiation
Sterility
Sterility testing
Sterilization
Sterilization - methods
Transplant Surgery
Ultraviolet radiation
Ultraviolet Rays
γ Radiation
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Summary:The presence of bone morphogenetic proteins in demineralized freeze-dried bone allograft (DFDBA) are responsible for developing hard tissues in intraosseous defects. The most common mode of sterilization of bone allografts, i.e., Gamma rays, have dramatic effects on the structural and biological properties of DFDBA, leading to loss of BMPs. Ultraviolet-C radiation is a newer approach to sterilize biodegradable scaffolds, which is simple to use and ensures efficient sterilization. However, UV-C radiation has not yet been effectively studied to sterilize bone allografts. This study aimed to compare and evaluate the effectiveness of Gamma and Ultraviolet-C rays in sterilizing indigenously prepared DFDBA and assess their effect on the quantity of BMP-7 present in the allograft. DFDBA samples from non-irradiated, gamma irradiated, and UV-C irradiated groups were tested for BMP-7 level and samples sterilized with gamma and UV-C rays were analysed for sterility testing. The estimated mean BMP-7 level was highest in non-irradiated DFDBA samples, followed by UV-C irradiated, and the lowest in gamma irradiated samples. Our study concluded that UV-C rays effectively sterilized DFDBA as indicated by negative sterility test and comprised lesser degradation of BMP-7 than gamma irradiation.
ISSN:1389-9333
1573-6814
1573-6814
DOI:10.1007/s10561-023-10103-2