Caproic acid production from food waste using indigenous microbiota: Performance and mechanisms

• Published in: Bioresource technology 2023-11, Vol.387, p.129687-129687, Article 129687
• Main Authors: Tang, Jialing, Yang, Hao, Pu, Yunhui, Hu, Yisong, Huang, Jin, Jin, Ni, He, Xinrui, Wang, Xiaochang C.
• Format: Article
• Language: English
• Subjects: Caproic acid; Chain elongation; Food waste; Lactic acid fermentation; Microbial community
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2023.129687

[Display omitted] •Food waste fermentation without inoculum under different pH was investigated.•Hydrolysis and acidogenesis processes were promoted with the increase of pH.•Caproic acid was produced at pH 6 through lactate-based chain elongation.•Functional microbial communities were orientationally enriched during fermentation. Caproic acid (CA) production from food waste (FW) is a promising way for waste recycling, while the fermentation processes need further exploration. In this study, FW acidogenic fermentation under different pH (uncontrolled, 4, 5, 6) using indigenous microbiota was investigated. Result showed that substrate hydrolysis, carbohydrate degradation and acidogenesis increased with the increase of pH. Although various microbial communities were observed in FW, lactic acid bacteria (Lactobacillus and Limosilactobacillus) were enriched at pH lower than 6, resulting in lactic acid accumulation. CA (88.24 mM) was produced at pH 6 accounting for 31.23% of the total product carbon. The enriched lactic acid bacteria were directionally replaced by chain elongators (Caproicibacter, Clostridium_sensu_stricto, unclassified_Ruminococcaceae) at pH 6, and carbohydrates in FW were firstly transformed into lactic acid, then to butyrate and CA through lactate-based chain elongation processes. This work provided a novel CA fermentation pathway and further enriched the FW valorization.