Catalytic Hairpin Assembly-Enhanced Graphene Transistor for Ultrasensitive miRNA Detection

MicroRNAs (miRNAs) have emerged as powerful biomarkers for disease diagnosis and screening. Traditional miRNA analytical techniques are inadequate for point-of-care testing due to their reliance on specialized expertise and instruments. Graphene field-effect transistors (GFETs) offer the prospect of...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2023-09, Vol.95 (35), p.13281-13288
Main Authors: Yang, Yuetong, Kong, Derong, Wu, Yungen, Chen, Yiheng, Dai, Changhao, Chen, Chang, Zhao, Junhong, Luo, Shi, Liu, Wentao, Liu, Yunqi, Wei, Dacheng
Format: Article
Language:English
Subjects:
Analytical chemistry
Assembly
Biomarkers
Biosensors
Chemistry
Diagnosis
DNA probes
Field effect transistors
Graphene
Lysates
MicroRNAs
miRNA
Nucleic acids
Probes
Semiconductor devices
Single-stranded DNA
Stability analysis
Synergistic effect
Transistors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:MicroRNAs (miRNAs) have emerged as powerful biomarkers for disease diagnosis and screening. Traditional miRNA analytical techniques are inadequate for point-of-care testing due to their reliance on specialized expertise and instruments. Graphene field-effect transistors (GFETs) offer the prospect of simple and label-free diagnostics. Herein, a GFET biosensor based on tetrahedral DNA nanostructure (TDN)-assisted catalytic hairpin assembly (CHA) reaction (TCHA) has been fabricated and applied to the sensitive and specific detection of miRNA-21. TDN structures are assembled to construct the biosensing interface, facilitating CHA reaction by providing free space and preventing unwanted entanglements, aggregation, and adsorption of probes on the graphene channel. Owing to synergistic effects of TDN-assisted in situ nucleic acid amplification on the sensing surface, as well as inherent signal sensitization of GFETs, the biosensor exhibits ultrasensitive detection of miRNA-21 down to 5.67 × 10–19 M, approximately three orders of magnitude lower than that normally achieved by graphene transistors with channel functionalization of single-stranded DNA probes. In addition, the biosensor demonstrates excellent analytical performance regarding selectivity, stability, and reproducibility. Furthermore, the practicability of the biosensor is verified by analyzing targets in a complex serum environment and cell lysates, showing tremendous potential in bioanalysis and clinical diagnosis.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.3c02433