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Thermal Adaptation Alters Response to Thermal Stress and Expression of Virulent Genes (eae, stx1, stx2, and hlyA) in Pathogenic Escherichia coli Isolated from Pork
Escherichia coli encounter variety of environmental and processing stresses during their growth, survival, and infection. Herein, the thermotolerance behavior and transcription of virulent genes responsible for the pathogenicity in isolated strains of pathogenic E. coli were evaluated. Among 176 E....
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| Published in: | Current microbiology 2023-10, Vol.80 (10), p.330-330, Article 330 |
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| creator | Singha, Songeeta Thomas, Rajendran Vishwakarma, Jai N. Bharadwaj, Devarshi Gupta, Vivek K. |
| description | Escherichia coli
encounter variety of environmental and processing stresses during their growth, survival, and infection. Herein, the thermotolerance behavior and transcription of virulent genes responsible for the pathogenicity in isolated strains of pathogenic
E. coli
were evaluated. Among 176
E. coli
isolates, 4 isolates (2.27%) were confirmed to be pathogenic
E. coli,
out of which 2 isolates were positive for EHEC and 2 were positive for EPEC based on their virulence factors. Thermotolerance was induced under thermal adaptation at higher temperature, regardless of the pathotypes. Cells grown and adapted at 42 °C, exhibited highest transcription of genes associated with adhesion (
eae
), hemolysis (
hly
A), and shiga toxin production (
stx
1)
.
However, expression of these genes was downregulated in cells adapted at lower temperature of 4 °C and 25 °C compared to control. Further, transcription of
stx2
was upregulated by 70% and 17% at 4 °C and 25 °C, respectively, while the transcription level was reduced by 44% relative to control at 42 °C. The findings indicate that expression of virulent genes in pathogenic
E. coli
at elevated temperature do not be depend on thermotolerance of the strain harboring these genes. |
| doi_str_mv | 10.1007/s00284-023-03446-2 |
| format | article |
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encounter variety of environmental and processing stresses during their growth, survival, and infection. Herein, the thermotolerance behavior and transcription of virulent genes responsible for the pathogenicity in isolated strains of pathogenic
E. coli
were evaluated. Among 176
E. coli
isolates, 4 isolates (2.27%) were confirmed to be pathogenic
E. coli,
out of which 2 isolates were positive for EHEC and 2 were positive for EPEC based on their virulence factors. Thermotolerance was induced under thermal adaptation at higher temperature, regardless of the pathotypes. Cells grown and adapted at 42 °C, exhibited highest transcription of genes associated with adhesion (
eae
), hemolysis (
hly
A), and shiga toxin production (
stx
1)
.
However, expression of these genes was downregulated in cells adapted at lower temperature of 4 °C and 25 °C compared to control. Further, transcription of
stx2
was upregulated by 70% and 17% at 4 °C and 25 °C, respectively, while the transcription level was reduced by 44% relative to control at 42 °C. The findings indicate that expression of virulent genes in pathogenic
E. coli
at elevated temperature do not be depend on thermotolerance of the strain harboring these genes.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-023-03446-2</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adaptation ; adhesion ; Biomedical and Life Sciences ; Biotechnology ; E coli ; Escherichia coli ; Gene expression ; Genes ; heat tolerance ; hemolysis ; High temperature ; Life Sciences ; Low temperature ; Microbiology ; Pathogenicity ; Pathogens ; pathotypes ; pork ; Shiga toxin ; temperature ; Temperature tolerance ; Thermal stress ; Toxins ; Transcription ; Virulence ; Virulence factors</subject><ispartof>Current microbiology, 2023-10, Vol.80 (10), p.330-330, Article 330</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c336t-ece0fc9decc5627b822635745efc3692d56fe290cec9fc4cb6fee6b7e7fa3c9e3</cites><orcidid>0000-0002-0405-8280</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>Singha, Songeeta</creatorcontrib><creatorcontrib>Thomas, Rajendran</creatorcontrib><creatorcontrib>Vishwakarma, Jai N.</creatorcontrib><creatorcontrib>Bharadwaj, Devarshi</creatorcontrib><creatorcontrib>Gupta, Vivek K.</creatorcontrib><title>Thermal Adaptation Alters Response to Thermal Stress and Expression of Virulent Genes (eae, stx1, stx2, and hlyA) in Pathogenic Escherichia coli Isolated from Pork</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><description>Escherichia coli
encounter variety of environmental and processing stresses during their growth, survival, and infection. Herein, the thermotolerance behavior and transcription of virulent genes responsible for the pathogenicity in isolated strains of pathogenic
E. coli
were evaluated. Among 176
E. coli
isolates, 4 isolates (2.27%) were confirmed to be pathogenic
E. coli,
out of which 2 isolates were positive for EHEC and 2 were positive for EPEC based on their virulence factors. Thermotolerance was induced under thermal adaptation at higher temperature, regardless of the pathotypes. Cells grown and adapted at 42 °C, exhibited highest transcription of genes associated with adhesion (
eae
), hemolysis (
hly
A), and shiga toxin production (
stx
1)
.
However, expression of these genes was downregulated in cells adapted at lower temperature of 4 °C and 25 °C compared to control. Further, transcription of
stx2
was upregulated by 70% and 17% at 4 °C and 25 °C, respectively, while the transcription level was reduced by 44% relative to control at 42 °C. The findings indicate that expression of virulent genes in pathogenic
E. coli
at elevated temperature do not be depend on thermotolerance of the strain harboring these genes.</description><subject>Adaptation</subject><subject>adhesion</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Gene expression</subject><subject>Genes</subject><subject>heat tolerance</subject><subject>hemolysis</subject><subject>High temperature</subject><subject>Life Sciences</subject><subject>Low temperature</subject><subject>Microbiology</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>pathotypes</subject><subject>pork</subject><subject>Shiga toxin</subject><subject>temperature</subject><subject>Temperature tolerance</subject><subject>Thermal stress</subject><subject>Toxins</subject><subject>Transcription</subject><subject>Virulence</subject><subject>Virulence 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Response to Thermal Stress and Expression of Virulent Genes (eae, stx1, stx2, and hlyA) in Pathogenic Escherichia coli Isolated from Pork</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><date>2023-10-01</date><risdate>2023</risdate><volume>80</volume><issue>10</issue><spage>330</spage><epage>330</epage><pages>330-330</pages><artnum>330</artnum><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>Escherichia coli
encounter variety of environmental and processing stresses during their growth, survival, and infection. Herein, the thermotolerance behavior and transcription of virulent genes responsible for the pathogenicity in isolated strains of pathogenic
E. coli
were evaluated. Among 176
E. coli
isolates, 4 isolates (2.27%) were confirmed to be pathogenic
E. coli,
out of which 2 isolates were positive for EHEC and 2 were positive for EPEC based on their virulence factors. Thermotolerance was induced under thermal adaptation at higher temperature, regardless of the pathotypes. Cells grown and adapted at 42 °C, exhibited highest transcription of genes associated with adhesion (
eae
), hemolysis (
hly
A), and shiga toxin production (
stx
1)
.
However, expression of these genes was downregulated in cells adapted at lower temperature of 4 °C and 25 °C compared to control. Further, transcription of
stx2
was upregulated by 70% and 17% at 4 °C and 25 °C, respectively, while the transcription level was reduced by 44% relative to control at 42 °C. The findings indicate that expression of virulent genes in pathogenic
E. coli
at elevated temperature do not be depend on thermotolerance of the strain harboring these genes.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s00284-023-03446-2</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-0405-8280</orcidid></addata></record> |
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| ispartof | Current microbiology, 2023-10, Vol.80 (10), p.330-330, Article 330 |
| issn | 0343-8651 1432-0991 |
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| source | Springer Nature |
| subjects | Adaptation adhesion Biomedical and Life Sciences Biotechnology E coli Escherichia coli Gene expression Genes heat tolerance hemolysis High temperature Life Sciences Low temperature Microbiology Pathogenicity Pathogens pathotypes pork Shiga toxin temperature Temperature tolerance Thermal stress Toxins Transcription Virulence Virulence factors |
| title | Thermal Adaptation Alters Response to Thermal Stress and Expression of Virulent Genes (eae, stx1, stx2, and hlyA) in Pathogenic Escherichia coli Isolated from Pork |
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