Cellular and bioenergetic effects of polystyrene microplastic in function of cell type, differentiation status and post-exposure time

• Published in: Environmental pollution (1987) 2023-11, Vol.337, p.122550-122550, Article 122550
• Main Authors: Peng, Miao, Vercauteren, Maaike, Grootaert, Charlotte, Rajkovic, Andreja, Boon, Nico, Janssen, Colin, Asselman, Jana
• Format: Article
• Language: English
• Subjects: Bioenergetic effects; Cell differentiation; Long-term impacts; Low particle concentrations; Microplastics
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2023.122550

The ubiquity of microplastics (MPs) in food sources and personal care products increasingly raises concerns on human health. However, little is known about the duration of the effects of MPs and whether effects depend on cellular differentiation status. Herein, cellular and bioenergetic effects of MPs in different exposure scenarios on four types of human cell lines derived from lung (A549 and BEAS-2B), colon (Caco-2) and liver (HepG2) were investigated. These cell lines are models for the major exposure routes in the body (inhalation, ingestion and physiological transport through the liver by the portal vein). To this aim, different scenarios were implemented by exposing undifferentiated and differentiated cells to single dosing of 2-μm polystyrene (PS) (102-105 particles/mL) for 48 h and 12 days. The undifferentiated Caco-2 cells with short exposure (48 h) showed the highest uptake rate of PS yet without significant cellular and mitochondrial responses. The biological effects, with the exception of ROS production, were not influenced by differentiation states of A549 and Caco-2 cells although differentiated cells showed much weaker ability to internalize PS. However, PS had significantly long-term impacts on cellular and mitochondrial functions even after the initial exposure period. In particular, Caco-2 cells that were post-exposed for 12 days after single PS dosing suffered higher oxidative stress and exhibited mitochondrial dysfunction than that for short exposure. Correspondingly, we observed that PS particles still remained in cell membrane and even in nuclei with high retention rate by 14-d post exposure during which metabolism and exchange of internalization and release occurred in cells. This indicates PS could induce chronic stress and even harmful effects on human cells after single intake that persists for a long time. This study paves the way for assessing the influence of PS on human health at low particle concentrations and with multiple exposure scenarios. [Display omitted] •Undifferentiated Caco-2 cells showed highest PS uptake rate among four cell lines.•Differentiated cells showed lower PS uptake rate than the undifferentiated.•Short PS exposure did not induce effects on cellular and mitochondrial functions.•PS evoked oxidative stress and mitochondrial dysfunction after 12-d post exposure.