Mechanisms of cardiac collapse at high temperature in a marine teleost (Girella nigrians)

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Molecular & integrative physiology, 2023-12, Vol.286, p.111512-111512, Article 111512
• Main Authors: Schwieterman, Gail D., Hardison, Emily A., Cox, Georgina K., Van Wert, Jacey C., Birnie-Gauvin, Kim, Eliason, Erika J.
• Format: Article
• Language: English
• Subjects: Arrhenius breakpoint temperature; Arrhythmia; Hyperkalemia; Hyperoxia; Hypoxia; Thermal performance curve
• ISSN: 1095-6433; 1531-4332
• DOI: 10.1016/j.cbpa.2023.111512

Heat-induced mortality in ectotherms may be attributed to impaired cardiac performance, specifically a collapse in maximum heart rate (fHmax), although the physiological mechanisms driving this phenomenon are still unknown. Here, we tested two proposed factors which may restrict cardiac upper thermal limits: noxious venous blood conditions and oxygen limitation. We hypothesized elevated blood [K+] (hyperkalemia) and low oxygen (hypoxia) would reduce cardiac upper thermal limits in a marine teleost (Girella nigricans), while high oxygen (hyperoxia) would increase thermal limits. We also hypothesized higher acclimation temperatures would exacerbate the harmful effects of an oxygen limitation. Using the Arrhenius breakpoint temperature test, we measured fHmax in acutely warmed fish under control (saline injected) and hyperkalemic conditions (elevated plasma [K+]) while exposed to hyperoxia (200% air saturation), normoxia (100% air saturation), or hypoxia (20% air saturation). We also measured ventricle lactate content and venous blood oxygen partial pressure (PO2) to determine if there were universal thresholds in either metric driving cardiac collapse. Elevated [K+] was not significantly correlated with any cardiac thermal tolerance metric. Hypoxia significantly reduced cardiac upper thermal limits (Arrhenius breakpoint temperature [TAB], peak fHmax, temperature of peak heart rate [TPeak], and temperature at arrhythmia [TARR]). Hyperoxia did not alter cardiac thermal limits compared to normoxia. There was no evidence of a species-wide threshold in ventricular [lactate] or venous PO2. Here, we demonstrate that oxygen limits cardiac thermal tolerance only in instances of hypoxia, but that other physiological processes are responsible for causing temperature-induced heart failure when oxygen is not limited. [Display omitted] •Thermal tolerance metrics for fHmax did not differ under hyperoxia and normoxia.•Hypoxia reduced thermal tolerance metrics for fHmax.•There were no threshold values for ventricle lactate at the time of arrhythmia.•There were no threshold values for venous PO2 at the time of arrhythmia.