Macrophage-expressed SRA ameliorates alcohol-induced liver injury by suppressing S-glutathionylation of Notch1 via recruiting thioredoxin

Scavenger receptor A (SRA) is preferentially expressed in macrophages and implicated as a multifunctional pattern recognition receptor for innate immunity. Hepatic macrophages play a primary role in the pathogenesis of alcoholic liver disease. Herein, we observed that SRA expression was significantl...

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Bibliographic Details
Published in:Journal of leukocyte biology 2024-01, Vol.115 (2), p.322-333
Main Authors: Li, Lei, Luo, Jialiang, Zhu, Zhengyumeng, Wang, Ping, Xu, Qishan, Chang, Bo, Wang, Di, Yu, Lu, Lu, Xiao, Zhou, Jia, Chen, Qingyun, Zuo, Daming
Format: Article
Language:English
Subjects:
Animals
Chemical and Drug Induced Liver Injury, Chronic - metabolism
Ethanol - toxicity
Immunologic Factors
Liver - metabolism
Macrophages - metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Receptors, Scavenger - metabolism
Scavenger Receptors, Class A - metabolism
Thioredoxins - genetics
Thioredoxins - metabolism
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Summary:Scavenger receptor A (SRA) is preferentially expressed in macrophages and implicated as a multifunctional pattern recognition receptor for innate immunity. Hepatic macrophages play a primary role in the pathogenesis of alcoholic liver disease. Herein, we observed that SRA expression was significantly increased in the liver tissues of mice with alcohol-related liver injury. SRA-deficient (SRA-/-) mice developed more severe alcohol-induced liver disease than wild-type mice. Enhanced liver inflammation existed in alcohol-challenged SRA-/- mice and was associated with increased Notch activation in hepatic macrophages compared with wild-type control animals. Mechanistically, SRA directly bound with Notch1 and suppressed its S-glutathionylation, thereby inhibiting Notch pathway activation. Further, we determined that the SRA interacted with thioredoxin-1 (Trx-1), a redox-active protein. SRA inhibited Trx-1 dimerization and facilitated the interaction of Trx-1 with Notch1. Application of a Trx-1-specific inhibitory agent during macrophage stimulation abolished SRA-mediated regulation of the Notch pathway and its downstream targets. In summary, our study revealed that SRA plays a critical role in macrophage inflammatory response by targeting Notch1 for its glutathionylation. SRA-mediated negative regulation of Notch activation might serve as a novel therapeutic strategy for alcohol-induced liver injury.
ISSN:1938-3673
1938-3673
DOI:10.1093/jleuko/qiad110