Genotype specific and microbiome effects of hypoxia in the model organism Daphnia magna

• Published in: Journal of evolutionary biology 2023-12, Vol.36 (12), p.1669-1683
• Main Authors: Coone, Manon, Bisschop, Karen, Vanoverberghe, Isabel, Verslype, Chris, Decaestecker, Ellen
• Format: Article
• Language: English
• Subjects: Climate change; Crustaceans; Daphnia magna; Donors; Environmental stress; Exposure; Freshwater crustaceans; Genotype & phenotype; Genotypes; genotype × microbiome × environmental interactions; global change; Hypoxia; Immunological tolerance; Intestinal microflora; microbiome transplants; Microbiomes; microbiome‐mediated phenotypic plasticity; Microbiota; Microorganisms; Survival; Transplantation; Transplants & implants
• ISSN: 1010-061X; 1420-9101
• DOI: 10.1111/jeb.14233

The fitness of the host is highly influenced by the interplay between the host and its associated microbiota. The flexible nature of these microbiota enables them to respond swiftly to shifts in the environment, which plays a key role in the host's capacity to withstand environmental stresses. To understand the role of the microbiome in host tolerance to hypoxia, one of the most significant chemical changes occurring in water ecosystems due to climate change, we performed a reciprocal gut transplant experiment with the freshwater crustacean Daphnia magna. In a microbiome transplant experiment, two genotypes of germ‐free recipients were inoculated with gut microbiota from Daphnia donors of their own genotype or from the other genotype, that had been either pre‐exposed to normoxic or hypoxic conditions. We found that D. magna individuals had a higher survival probability in hypoxia if their microbiome had been pre‐exposed to hypoxia. The bacterial communities of the recipients changed over time with a reduction in alpha diversity, which was stronger when donors were pre‐exposed to a hypoxic environment. While donor genotype had no influence on the long‐term survival probability in hypoxia, donor genotypes was the most influential factor of the microbial community 3 days after the transplantation. Our results indicate that microbiome influencing factors mediate host fitness in a hypoxic environment in a time depending way. The fitness of the host is highly influenced by the interplay between the host and its associated microbiota. The flexible nature of these microbiota enables them to respond quickly to shifts in the environment, which plays a key role in the host's capacity to withstand environmental stresses. To understand the role of the microbiome in host tolerance to hypoxia, one of the most significant chemical changes occurring in water ecosystems due to climate change, we performed a reciprocal gut microbiome transplant experiment with the fresh water crustacean Daphnia magna. Two genotypes of germ‐free recipients were inoculated with gut microbiota from Daphnia donors of their own genotype or from the other genotype, that had been either pre‐exposed to normoxic or hypoxic conditions. We found that Daphnia magna individuals had a higher survival probability in hypoxia if their microbiome had been pre‐exposed to hypoxia, moreover the bacterial communities of the recipients changed over time indicating that the microbiome mediates host fitness in a hyp