A noninvasive multianalytical approach establishment for risk assessment and gastric cancer screening

Effective screening and early detection are critical to improve the prognosis of gastric cancer (GC). Our study aims to explore noninvasive multianalytical biomarkers and construct integrative models for preliminary risk assessment and GC detection. Whole genomewide methylation marker discovery was...

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Bibliographic Details
Published in:International journal of cancer 2024-03, Vol.154 (6), p.1111-1123
Main Authors: Fan, Xiao‐Han, Zhang, Yang, Wang, Pei, Song, Qian‐Qian, Wang, Mona, Mejias‐Luque, Raquel, Li, Zhe‐Xuan, Zhou, Tong, Zhang, Jing‐Ying, Liu, Wei‐Dong, Zhang, Lan‐Fu, Li, Wen‐Qing, You, Wei‐Cheng, Gerhard, Markus, Jiao, Yu‐Chen, Wang, Xiao‐Bing, Pan, Kai‐Feng
Format: Article
Language:English
Subjects:
biomarker
Biomarkers
Biomarkers, Tumor - genetics
Cancer
Cancer screening
CpG Islands
DNA Methylation
Early Detection of Cancer
Gastric cancer
Genomes
Health risk assessment
Helicobacter Infections - diagnosis
Helicobacter Infections - genetics
Helicobacter Infections - pathology
Helicobacter pylori
Helicobacter pylori - genetics
Humans
Medical prognosis
Medical research
Medical screening
methylation
Mutation
precancerous lesions
Risk Assessment
Stomach Neoplasms - diagnosis
Stomach Neoplasms - genetics
Stomach Neoplasms - pathology
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Summary:Effective screening and early detection are critical to improve the prognosis of gastric cancer (GC). Our study aims to explore noninvasive multianalytical biomarkers and construct integrative models for preliminary risk assessment and GC detection. Whole genomewide methylation marker discovery was conducted with CpG tandems target amplification (CTTA) in cfDNA from large asymptomatic screening participants in a high‐risk area of GC. The methylation and mutation candidates were validated simultaneously using one plasma from patients at various gastric lesion stages by multiplex profiling with Mutation Capsule Plus (MCP). Helicobacter pylori specific antibodies were detected with a recomLine assay. Integrated models were constructed and validated by the combination of multianalytical biomarkers. A total of 146 and 120 novel methylation markers were found in CpG islands and promoter regions across the genome with CTTA. The methylation markers together with the candidate mutations were validated with MCP and used to establish a 133‐methylation‐marker panel for risk assessment of suspicious precancerous lesions and GC cases and a 49‐methylation‐marker panel as well as a 144‐amplicon‐mutation panel for GC detection. An integrated model comprising both methylation and specific antibody panels performed better for risk assessment than a traditional model (AUC, 0.83 and 0.63, P 
ISSN:0020-7136
1097-0215
DOI:10.1002/ijc.34739