Ultrasound-Targeted Microbubble Destruction Assisted Delivery of Platelet-Rich Plasma-Derived Exosomes Promoting Peripheral Nerve Regeneration

Peripheral nerve injury is prevalent and has a high disability rate in clinical settings. Current therapeutic methods have not achieved satisfactory efficacy, underscoring the need for novel approaches to nerve restoration that remains an active area of research in neuroscience and regenerative medi...

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Bibliographic Details
Published in:Tissue engineering. Part A 2023-12, Vol.29 (23-24), p.645-662
Main Authors: Yi, Dan, Zhang, Yongyi, Li, Molin, Chen, Jian, Chen, Xianghui, Wang, Li, Xing, Guanghui, Chen, Siming, Zhu, Yaqiong, Wang, Yuexiang
Format: Article
Language:English
Subjects:
Animals
Exosomes - metabolism
Microbubbles
Nerve Regeneration
Original Articles
Peripheral Nerve Injuries - metabolism
Peripheral Nerve Injuries - therapy
Platelet-Rich Plasma
Rats
Schwann Cells
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Summary:Peripheral nerve injury is prevalent and has a high disability rate in clinical settings. Current therapeutic methods have not achieved satisfactory efficacy, underscoring the need for novel approaches to nerve restoration that remains an active area of research in neuroscience and regenerative medicine. In this study, we isolated platelet-rich plasma-derived exosomes (PRP-exos) and found that they can significantly enhance the proliferation, migration, and secretion of trophic factors by Schwann cells (SCs). In addition, there were marked changes in transcriptional and expression profiles of SCs, particularly via the upregulation of genes related to biological functions involved in nerve regeneration and repair. In the rat model of sciatic nerve crush injury, ultrasound-targeted microbubble destruction (UTMD) enhanced the efficiency of PRP-exos delivery to the injury site. This approach ensured a high concentration of PRP-exos in the injured nerve and improved the therapeutic outcomes. In conclusion, PRP-exos may promote nerve regeneration and repair, and UTMD may increase the effectiveness of targeted PRP-exos delivery to the injured nerve and enhance the therapeutic effect. Color images are available online.
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2023.0133