The impact of leucine supplementation on body composition and glucose tolerance following energy restriction: an 8-week RCT in adults at risk of the metabolic syndrome

• Published in: European journal of clinical nutrition 2024-02, Vol.78 (2), p.155-162
• Main Authors: Pathak, Kaveri, Zhao, Yun, Calton, Emily K., James, Anthony P., Newsholme, Philip, Sherriff, Jill, Soares, Mario J.
• Format: Article
• Language: English
• Subjects: 631/443/319; 692/308/53; Adults; Body composition; Body fat; Clinical Nutrition; Clinical trials; Confidence intervals; Dietary restrictions; Dietary supplements; Dual energy X-ray absorptiometry; Energy; Epidemiology; Fat-free body mass; Glucose; Glucose metabolism; Glucose tolerance; Insulin; Internal Medicine; Lactose; Leucine; Medicine; Medicine & Public Health; Men; Metabolic Diseases; Metabolic disorders; Metabolic syndrome; Nutrient deficiency; Parameter estimation; Public Health; Supplements
• ISSN: 0954-3007; 1476-5640
• DOI: 10.1038/s41430-023-01360-1

Background L-Leucine (Leu) supplementation may benefit fat-free mass (FFM) per se and glucose metabolism. Objectives To determine whether Leu supplementation during energy restriction blunted the loss of FFM, enhanced the loss of fat mass (FM) and improved glucose tolerance. Design Thirty-seven adults, aged 20–65 years, with increased waist circumference and at least one other metabolic syndrome (MetS) component, were selected. We employed a two-arm parallel, double blind, randomized control trial (RCT) design. Participants were randomly assigned to an intervention group (leucine – 3 g/d) or placebo (lactose - 2.67 g/d), while following an individualised energy restricted diet for an 8-week period. Detailed body composition (DEXA), oral glucose tolerance test (OGTT), insulin and components of MetS were measured before and after the trial. Analysis of covariance (ANCOVA) assessed the effect of Leu on an intention-to-treat (ITT) principle. Bootstrapping method with 1000 bootstrap samples was used to derive parameter estimates, standard errors, p -values, and 95% confidence intervals for all outcomes. Results Adjusted for baseline values and other covariates, FFM ( p  = 0.045) and lean tissue mass (LTM) ( p  = 0.050) were significantly higher following Leu. These outcomes were modified by a significant treatment x sex interaction that indicated Leu had the greater effect in men. However, on adjustment for body composition changes, there was no difference in insulin sensitivity, oral glucose tolerance, or change in MetS components following Leu. Conclusion Short-term leucine supplementation during energy restriction resulted in a greater preservation of FFM and LTM particularly in men, but did not impact glucose metabolism.