Presence of leukemic clone‐specific immunoglobulin heavy chain rearrangements in neonatal blood spots of children with B‐cell precursor acute lymphoblastic leukemia

• Published in: International journal of laboratory hematology 2024-04, Vol.46 (2), p.303-311
• Main Authors: Kacanski, Natasa, Kolarovic, Jovanka, Kostic, Tatjana, Marjanovic, Irena, Janic, Dragana, Pavlovic, Sonja, Karan‐Djurasevic, Teodora
• Format: Article
• Language: English
• Subjects: Acute lymphoblastic leukemia; Birth weight; Blood; Bone marrow; Child; Child, Preschool; childhood acute lymphoblastic leukemia; Children; Chromosome aberrations; Clone Cells; Cloning; Diagnosis; Female; Fusion protein; Gene Rearrangement; Guthrie cards; Heavy chains; Humans; immunoglobulin heavy chain rearrangements; Immunoglobulin Heavy Chains - genetics; Immunoglobulins; Infant, Newborn; Leukemia; Leukocytes; Lymphatic leukemia; Neonates; Patients; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - diagnosis; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - genetics; Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics; Pregnancy; prenatal origin; Retrospective Studies; Runx1 protein
• ISSN: 1751-5521; 1751-553X
• DOI: 10.1111/ijlh.14200

Introduction Childhood B‐cell precursor acute lymphoblastic leukemia (BCP‐ALL) can be traced back to birth using leukemic clone‐specific immunoglobulin heavy chain (IGH) rearrangements, implying prenatal origin of this disease. Methods We retrospectively analyzed neonatal blood spots (Guthrie cards) of 24 patients with childhood BCP‐ALL aged 1–9.6 years (median 3.1 years) for the presence of clonotypic IGH rearrangements identified in diagnostic bone marrow samples. Based on the sequences of IGH rearrangements, 2 patient‐specific primers were designed for each patient and used in semi‐nested polymerase chain reaction for the detection of preleukemic clones at birth. Results Clonotypic IGH rearrangements were detected in neonatal blood spots of 54.2% of patients (13/24). In two cases with double IGH rearrangements detected at diagnosis, only one rearrangement was present at birth, while in the third case both leukemic rearrangements were detected in neonatal blood. Guthrie card‐positive findings were significantly more frequent in children ≤5 years of age than in older children (p = 0.011). Regarding patients' characteristics at birth and at diagnosis, Guthrie card‐positivity was not associated with sex, birth weight and mother's age, as well as with white blood cell count, percentage of bone marrow blasts, immunophenotype and the presence of ETV6/RUNX1 and TCF3/PBX1 fusion genes at diagnosis. Conclusion Our study confirms that a large proportion of childhood BCP‐ALL originates in utero, regardless of the molecular subtype defined by chromosomal aberrations. The observed trend toward younger age at diagnosis in Guthrie card‐positive versus Guthrie card‐negative patients implies that the age at diagnosis depends on the presence of preleukemic clone at birth, as well as on the timing of postnatal transforming genetic events.