Evaluation of simple strategies to reduce the use of antibiotics in equine fresh sperm

According to the World Health Organization in 2021, the development of antimicrobial resistances is considered one of the top ten global public health threats. Microbial load negatively affects sperm quality and preservation (Ortega-Ferrusola and Peña, Reproduction in Domestic Animals. 2009; 44(3):5...

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Bibliographic Details
Published in:Journal of equine veterinary science 2023-06, Vol.125, p.104628, Article 104628
Main Authors: Zabala, Sonsoles M, Gutiérrez-Cepeda, Luna, Lorenzo, Pedro L, Pérez-Aguilera, Verónica, Carneiro, Gustavo F, Moreno, Santiago, Domínguez-Gimbernat, Mónica, Montero, Natalia, González-Zorn, Bruno, Crespo, Francisco, Serres, Consuelo
Format: Article
Language:English
Subjects:
adults
agar
antibiotics
centrifugation
culture media
filtration
glucose
horses
hygiene
males
microbial load
phylloquinone
pollution
protocols
public health
semen
sperm motility
sperm quality
statistical analysis
veterinary medicine
viability
World Health Organization
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Summary:According to the World Health Organization in 2021, the development of antimicrobial resistances is considered one of the top ten global public health threats. Microbial load negatively affects sperm quality and preservation (Ortega-Ferrusola and Peña, Reproduction in Domestic Animals. 2009; 44(3):518-522), which implies the use of antibiotics in semen extenders. The objective of this study was to study the effect of three semen processing techniques on quality and microbial load of fresh equine sperm to evaluate the possibility of reducing or eliminating the prophylactic use of antibiotics in the future. Ten adult stallions were included (one ejaculate per male). To avoid environmental contamination during sperm collection and processing, a protocol that maximizes hygiene measures was implemented. Aliquots of each ejaculate were processed following 4 protocols: Simple Centrifugation (450g x 7 min) in extender with antibiotics (SC+, as control group), Simple Centrifugation in extender without antibiotics (SC-), Single-Layer Colloidal Centrifugation (300g x 20 min) in extender without antibiotic (CC-) and Filtration (SpermFilter®) in extender without antibiotics (F-). Sperm motility (total and progressive motility, TM and PM, respectively, %), viability (Alives, %), and microbial load (on three different culture media (Columbia 5% Sheep Blood Agar, COS, general purpose medium for non-fastidious and fastidiousmicroorganisms, Sabouraud Dextrose Agar, SDA, for yeasts and molds, and Schaedler vitamin K1 5% Sheep Blood Agar, SCH, for fastidious anaerobic bacteria) were evaluated after processing. Statistical analysis was performed by Kruskal-Wallis, ANOVA and post hoc tests (p
ISSN:0737-0806
1542-7412
DOI:10.1016/j.jevs.2023.104628