Phosphoproteome analyses pinpoint the F‐box protein SLOW MOTION as a regulator of warm temperature‐mediated hypocotyl growth in Arabidopsis

Summary Hypocotyl elongation is controlled by several signals and is a major characteristic of plants growing in darkness or under warm temperature. While already several molecular mechanisms associated with this process are known, protein degradation and associated E3 ligases have hardly been studi...

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Published in:The New phytologist 2024-01, Vol.241 (2), p.687-702
Main Authors: Zhu, Shanshuo, Pan, Lixia, Vu, Lam Dai, Xu, Xiangyu, Orosa‐Puente, Beatriz, Zhu, Tingting, Neyt, Pia, Cotte, Brigitte, Jacobs, Thomas B., Gendron, Joshua M., Spoel, Steven H., Gevaert, Kris, De Smet, Ive
Format: Article
Language:English
Subjects:
(phospho)proteomics
Ambient temperature
Arabidopsis
Arabidopsis - metabolism
Arabidopsis Proteins - metabolism
Biodegradation
Darkness
Degradation
DWARF1
Elongation
F-Box Proteins - metabolism
Gene Expression Regulation, Plant
Growth regulators
hypocotyl
Hypocotyl - metabolism
Molecular modelling
Phosphorylation
Proteins
Seedlings
Serine
SLOW MOTION
Substrates
Temperature
thermomorphogenesis
Transcription
Ubiquitin
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Summary:Summary Hypocotyl elongation is controlled by several signals and is a major characteristic of plants growing in darkness or under warm temperature. While already several molecular mechanisms associated with this process are known, protein degradation and associated E3 ligases have hardly been studied in the context of warm temperature. In a time‐course phosphoproteome analysis on Arabidopsis seedlings exposed to control or warm ambient temperature, we observed reduced levels of diverse proteins over time, which could be due to transcription, translation, and/or degradation. In addition, we observed differential phosphorylation of the LRR F‐box protein SLOMO MOTION (SLOMO) at two serine residues. We demonstrate that SLOMO is a negative regulator of hypocotyl growth, also under warm temperature conditions, and protein–protein interaction studies revealed possible interactors of SLOMO, such as MKK5, DWF1, and NCED4. We identified DWF1 as a likely SLOMO substrate and a regulator of warm temperature‐mediated hypocotyl growth. We propose that warm temperature‐mediated regulation of SLOMO activity controls the abundance of hypocotyl growth regulators, such as DWF1, through ubiquitin‐mediated degradation.
ISSN:0028-646X
1469-8137
1469-8137
DOI:10.1111/nph.19383