NAMPT regulates mitochondria function and lipid metabolism during porcine oocyte maturation

Oocyte maturation defect can lead to maternal reproduction disorder. NAMPT is a rate‐limiting enzyme in mammalian NAD+ biosynthesis pathway, which can regulate a variety of cellular metabolic processes including glucose metabolism and DNA damage repair. However, the function of NAMPT in porcine oocy...

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Bibliographic Details
Published in:Journal of cellular physiology 2024-01, Vol.239 (1), p.180-192
Main Authors: Guo, Xin, Jiao, Le, Yi, Yang, Zhang, Hao‐Lin, Liu, Ya‐Xi, Wang, Zi‐Yu, Sun, Shao‐Chen
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Biosynthesis
Chromosomes
Defects
Deoxyribonucleic acid
DNA
DNA damage
DNA repair
Gametocytes
Glucose metabolism
lipid
Lipid metabolism
Lipid Metabolism - genetics
Lipids
Lipogenesis
Lipolysis
Mammals
Maturation
Meiosis
Membrane potential
Metabolism
Mitochondria
Mitochondria - metabolism
Mitochondrial DNA
NAMPT
oocyte
Oocytes
Oocytes - metabolism
Oogenesis
Oxidative Stress
spindle
Swine
Tubulin
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Summary:Oocyte maturation defect can lead to maternal reproduction disorder. NAMPT is a rate‐limiting enzyme in mammalian NAD+ biosynthesis pathway, which can regulate a variety of cellular metabolic processes including glucose metabolism and DNA damage repair. However, the function of NAMPT in porcine oocytes remains unknown. In this study, we showed that NAMPT involved into multiple cellular events during oocyte maturation. NAMPT expressed during all stages of porcine oocyte meiosis, and inhibition of NAMPT activity caused the cumulus expansion and polar body extrusion defects. Mitochondrial dysfunction was observed in NAMPT‐deficient porcine oocytes, which showed decreased membrane potential, ATP and mitochondrial DNA content, increased oxidative stress level and apoptosis. We also found that NAMPT was essential for spindle organization and chromosome arrangement based on Ac‐tubulin. Moreover, lack of NAMPT activity caused the increase of lipid droplet and affected the imbalance of lipogenesis and lipolysis. In conclusion, our study indicated that lack of NAMPT activity affected porcine oocyte maturation through its effects on mitochondria function, spindle assembly and lipid metabolism.
ISSN:0021-9541
1097-4652
1097-4652
DOI:10.1002/jcp.31156