Pumilio1 regulates NPM3/NPM1 axis to promote PD-L1-mediated immune escape in gastric cancer

• Published in: Cancer letters 2024-01, Vol.581, p.216498-216498, Article 216498
• Main Authors: Wang, Han, Zhou, Zhijun, Zhang, Junchang, Hao, Tengfei, Wang, Pengliang, Wu, Pei, Su, Rishun, Yang, Huan, Deng, Guofei, Chen, Songyao, Gu, Liang, He, Yulong, Zeng, Leli, Zhang, Changhua, Yin, Songcheng
• Format: Article
• Language: English
• Subjects: B7-H1 Antigen - genetics; B7-H1 Antigen - metabolism; CD8-Positive T-Lymphocytes; Cell Line, Tumor; Gastric cancer; Humans; Immune escape; NPM3; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Nucleoplasmins - metabolism; PD-L1; PUM1; RNA-Binding Proteins - genetics; Stomach Neoplasms - pathology; Tumor Microenvironment
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/j.canlet.2023.216498

Abnormal regulation of RNA binding proteins (RBPs) plays an essential role in tumorigenesis and progression, but their functions and mechanisms remain largely elusive. Previously, we reported that Pumilio 1 (PUM1), a RBP, could regulate glycolysis metabolism and promote the progression of gastric cancer (GC). However, the role of PUM1 in tumor immune regulation remains largely elusive. In this study, we report that PUM1 induces immune escape through posttranscriptional regulation of PD-L1 in GC. We used multiplexed immunohistochemistry to analyze the correlation between PUM1 expression and immune microenvironment in GC. The effect of PUM1 deficiency on tumor killing of T cells was examined in vitro and in vivo. The molecular mechanism of PUM1 was evaluated via RNA immunoprecipitation, chromatin immunoprecipitation, Western blot, co-immunoprecipitation, and RNA stability assays. Clinically, elevated PUM1 expression is associated with high-expression of PD-L1, lack of CD8+ T cell infiltration and poor prognosis in GC patients. PUM1 positively regulates PD-L1 expression and PUM1 reduction enhances T cell killing of tumors. Mechanistically, PUM1 directly binds to nucleophosmin/nucleoplasmin 3 (NPM3) mRNA and stabilizes NPM3. NPM3 interacts with NPM1 to promote NPM1 translocation into the nucleus and increase the transcription of PD-L1. PUM1 inhibits the anti-tumor activity of T cells through the PUM1/NPM3/PD-L1 axis. In summary, this study reveals the critical post-transcriptional effect of PUM1 in the modulation of PD-L1-dependent GC immune escape, thus provides a novel indicator and potential therapeutic target for cancer immunotherapy. •PUM1 positively regulates PD-L1 in GC•PUM1 binds NPM3 mRNA and positively regulates its expression•NPM3 interacted with NPM1 and regulated PD-L1 expression•PUM1/NPM3/PD-L1 axis can promote immune escape in GC