Dissecting embryonic and extraembryonic lineage crosstalk with stem cell co-culture

Embryogenesis necessitates harmonious coordination between embryonic and extraembryonic tissues. Although stem cells of both embryonic and extraembryonic origins have been generated, they are grown in different culture conditions. In this study, utilizing a unified culture condition that activates t...

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Bibliographic Details
Published in:Cell 2023-12, Vol.186 (26), p.5859-5875.e24
Main Authors: Wei, Yulei, Zhang, E, Yu, Leqian, Ci, Baiquan, Sakurai, Masahiro, Guo, Lei, Zhang, Xin, Lin, Sirui, Takii, Shino, Liu, Lizhong, Liu, Jian, Schmitz, Daniel A, Su, Ting, Zhang, Junmei, Shen, Qiaoyan, Ding, Yi, Zhan, Linfeng, Sun, Hai-Xi, Zheng, Canbin, Xu, Lin, Okamura, Daiji, Ji, Weizhi, Tan, Tao, Wu, Jun
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation
Cell Lineage
Coculture Techniques
Embryo, Mammalian
Embryonic Stem Cells - metabolism
Endoderm - metabolism
Macaca fascicularis
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Summary:Embryogenesis necessitates harmonious coordination between embryonic and extraembryonic tissues. Although stem cells of both embryonic and extraembryonic origins have been generated, they are grown in different culture conditions. In this study, utilizing a unified culture condition that activates the FGF, TGF-β, and WNT pathways, we have successfully derived embryonic stem cells (FTW-ESCs), extraembryonic endoderm stem cells (FTW-XENs), and trophoblast stem cells (FTW-TSCs) from the three foundational tissues of mouse and cynomolgus monkey (Macaca fascicularis) blastocysts. This approach facilitates the co-culture of embryonic and extraembryonic stem cells, revealing a growth inhibition effect exerted by extraembryonic endoderm cells on pluripotent cells, partially through extracellular matrix signaling. Additionally, our cross-species analysis identified both shared and unique transcription factors and pathways regulating FTW-XENs. The embryonic and extraembryonic stem cell co-culture strategy offers promising avenues for developing more faithful embryo models and devising more developmentally pertinent differentiation protocols.
ISSN:0092-8674
1097-4172
DOI:10.1016/j.cell.2023.11.008