SERS detection of foodborne pathogens in beverage with Au nanostars

The aim of this study is to develop a simple but rapid method for the determination of foodborne pathogens in complex matrices (beverages) by surface enhanced Raman spectroscopy (SERS) combined with Au nanostar solid-phase substrates. The star-shaped singlet Au nanostructure was formed on the surfac...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2024-01, Vol.191 (1), p.28-28, Article 28
Main Authors: Zeng, Pei, Guan, Qi, Zhang, Qianqian, Yu, Lili, Yan, Xianzai, Hong, Yanping, Duan, Luying, Wang, Chunrong
Format: Article
Language:English
Subjects:
Analytical Chemistry
Beverages
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Cluster analysis
Gold - chemistry
Metal Nanoparticles - chemistry
Metal sheets
Microengineering
Nanochemistry
Nanotechnology
Original Paper
Pathogens
Principal components analysis
Raman spectroscopy
Reproducibility of Results
Rhodamine 6G
Solid phases
Spectrum Analysis, Raman - methods
Stainless steels
Substrates
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Summary:The aim of this study is to develop a simple but rapid method for the determination of foodborne pathogens in complex matrices (beverages) by surface enhanced Raman spectroscopy (SERS) combined with Au nanostar solid-phase substrates. The star-shaped singlet Au nanostructure was formed on the surface of a stainless steel sheet by chemical replacement reaction. Rhodamine 6G verified the sensitivity and reproducibility of this substrate, and the relative standard deviations of the SERS intensity at 1312 cm −1 , 1364 cm −1 , and 1510 cm −1 displacements were 3.40%, 5.64%, and 3.48%, respectively. By detecting four pathogens in beverage samples on Au nanostar substrates, the utility of the SERS assay was demonstrated, while the combination of principal component analysis (PCA) and hierarchical cluster analysis (HCA) further enabled the isolation and identification of pathogens. The results of spiked beverages were validated in conventional culture identification and Vitek 2 Compact biochemical identification system experiments. Thus, this research demonstrated that Au nanostar substrates can be effectively utilized for the recognition of pathogenic bacteria and have immense promise to advance the progress of quick detection of foodborne pathogens and food safety. Graphical abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-023-06105-2