ATP-mediated increase in H + efflux from retinal Müller cells of the axolotl

• Published in: Journal of neurophysiology 2024-01, Vol.131 (1), p.124-136
• Main Authors: Kreitzer, Matthew A, Vredeveld, Mason, Tinner, Kaleb, Powell, Alyssa M, Schantz, Adam W, Leininger, Rachel, Merillat, Rajapone, Gongwer, Michael W, Tchernookova, Boriana K, Malchow, Robert Paul
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - metabolism; Ambystoma mexicanum - metabolism; Amiloride - metabolism; Animals; Calcium - metabolism; Calmodulin - metabolism; Ependymoglial Cells - metabolism; Neuroglia - metabolism; Retina
• ISSN: 0022-3077; 1522-1598; 1522-1598
• DOI: 10.1152/jn.00321.2023

Previous work has shown that activation of tiger salamander retinal radial glial cells by extracellular ATP induces a pronounced extracellular acidification, which has been proposed to be a potent modulator of neurotransmitter release. This study demonstrates that low micromolar concentrations of extracellular ATP similarly induce significant H effluxes from Müller cells isolated from the axolotl retina. Müller cells were enzymatically isolated from axolotl retina and H fluxes were measured from individual cells using self-referencing H -selective microelectrodes. The increased H efflux from axolotl Müller cells induced by extracellular ATP required activation of metabotropic purinergic receptors and was dependent upon calcium released from internal stores. We further found that the ATP-evoked increase in H efflux from Müller cells of both tiger salamander and axolotl were sensitive to pharmacological agents known to interrupt calmodulin and protein kinase C (PKC) activity: chlorpromazine (CLP), trifluoperazine (TFP), and W-7 (all calmodulin inhibitors) and chelerythrine, a PKC inhibitor, all attenuated ATP-elicited increases in H efflux. ATP-initiated H fluxes of axolotl Müller cells were also significantly reduced by amiloride, suggesting a significant contribution by sodium-hydrogen exchangers (NHEs). In addition, α-cyano-4-hydroxycinnamate (4-cin), a monocarboxylate transport (MCT) inhibitor, also reduced the ATP-induced increase in H efflux in both axolotl and tiger salamander Müller cells, and when combined with amiloride, abolished ATP-evoked increase in H efflux. These data suggest that axolotl Müller cells are likely to be an excellent model system to understand the cell-signaling pathways regulating H release from glia and the role this may play in modulating neuronal signaling. Glial cells are a key structural part of the tripartite synapse and have been suggested to regulate synaptic transmission, but the regulatory mechanisms remain unclear. We show that extracellular ATP, a potent glial cell activator, induces H efflux from axolotl retinal Müller (glial) cells through a calcium-dependent pathway that is likely to involve calmodulin, PKC, Na /H exchange, and monocarboxylate transport, and suggest that such H release may play a key role in modulating neuronal transmission.