Sequencing Support System: A robotic system for processing DNA samples

The development of very high-throughput and autonomous robotic workstations will be necessary to quickly and efficiently complete the sequencing of the 3 billion nucleotide base pairs that make up the human genome. The key to our sequencing scale-up efforts is the development of the Sequencing Suppo...

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Bibliographic Details
Published in:IEEE engineering in medicine and biology magazine 1997-03, Vol.16 (2), p.92-93
Main Author: Brignac, S.
Format: Article
Language:English
Subjects:
Assembly systems
Bioinformatics
Chromosome Mapping
Computer Systems
DNA
Finishing
Genomics
Humans
Rails
Robotics
Robots
Sequence Analysis, DNA
Sequences
Workstations
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Summary:The development of very high-throughput and autonomous robotic workstations will be necessary to quickly and efficiently complete the sequencing of the 3 billion nucleotide base pairs that make up the human genome. The key to our sequencing scale-up efforts is the development of the Sequencing Support System. The Sequencing Support System is an integrated robotic workstation built around a 3-meter rail robot. The Sequencing Support System is currently assembling cycle sequence reactions and thermal cycling and consolidating reactions. We are currently testing plaque picking and DNA purification methods that can be effectively and quickly integrated with our current platform. Our intent is to quickly develop and construct an autonomous high-throughput system that is replicatable for inclusive large-scale sequencing efforts. This integrated workstation will consist of the Sequencing Support System; a bank of automated sequencers; and a custom 192-channel oligo synthesizer, a vital instrument critical to labor intensive "finishing" efforts (sequencing methods directed at filling gaps and improving the quality of the produced DNA sequence). Each of these components are now operational as separate modules. Data transfer and assembly are automated in software on Mac and Sun computers. Our next system, currently in the planning phase, will be solely dedicated to automated high-throughput "finishing". The system will comprise the Sequencing Support System's components and will include a Beekman Biomek workstation specifically tasked to manipulate individual clones.
ISSN:0739-5175
1937-4186
DOI:10.1109/51.582189