Mechanism of enhanced microalgal biomass and lipid accumulation through symbiosis between a highly succinic acid-producing strain of Escherichia coli SUC and Aurantiochytrium sp. SW1

Mechanism of enhanced microalgal biomass and lipid accumulation through symbiosis between a highly succinic acid-producing strain of Escherichia coli SUC and Aurantiochytrium sp. SW1. [Display omitted] •E. coli SUC was screened to establish a symbiotic system with SW1.•E. coli SUC promoted the growt...

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Published in:Bioresource technology 2024-02, Vol.394, p.130232-130232, Article 130232
Main Authors: Zhang, Mei-Yu, Xu, Xin-Ru, Zhao, Ru-Ping, Huang, Chao, Song, Yuan-Da, Zhao, Zi-Tong, Zhao, Yu-Bin, Ren, Xiao-Jie, Zhao, Xin-He
Format: Article
Language:English
Subjects:
Algal-bacterial symbiosis
Aurantiochytrium
Docosahexaenoic acid (DHA)
Microalgae lipid
Regulatory mechanism
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Summary:Mechanism of enhanced microalgal biomass and lipid accumulation through symbiosis between a highly succinic acid-producing strain of Escherichia coli SUC and Aurantiochytrium sp. SW1. [Display omitted] •E. coli SUC was screened to establish a symbiotic system with SW1.•E. coli SUC promoted the growth of SW1 by dual regulation mechanism.•Under optimized conditions, SW1 achieved biomass of 27.83 g/L and lipids of 4.87 g/L. Microalgae, known for rapid growth and lipid richness, hold potential in biofuels and high-value biomolecules. The symbiotic link with bacteria is crucial in large-scale open cultures. This study explores algal-bacterial interactions using a symbiotic model, evaluating acid-resistant Lactic acid bacteria (LAB), stress-resilient Bacillus subtilis and Bacillus licheniformis, and various Escherichia coli strains in the Aurantiochytrium sp. SW1 system. It was observed that E. coli SUC significantly enhanced the growth and lipid production of Aurantiochytrium sp. SW1 by increasing enzyme activity (NAD-IDH, NAD-ME, G6PDH) while maintaining sustained succinic acid release. Optimal co-culture conditions included temperature 28 °C, a 1:10 algae-to-bacteria ratio, and pH 8. Under these conditions, Aurantiochytrium sp. SW1 biomass increased 3.17-fold to 27.83 g/L, and total lipid content increased 2.63-fold to 4.87 g/L. These findings have implications for more efficient microalgal lipid production and large-scale cultivation.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2023.130232