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A facile, low-cost bimetallic iron-nickel MOF nanozyme-propelled ratiometric fluorescent sensor for highly sensitive and selective uric acid detection and its smartphone application
As a kind of well-known disease biomarker, uric acid (UA) is closely associated with normal metabolism and health. Despite versatile nanozymes facilitating the analysis of UA, most previous works could only generate single-signal outputs with unsatisfactory detection performance. Exploring a novel r...
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Published in: | Nanoscale 2024-01, Vol.16 (3), p.1394-145 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | As a kind of well-known disease biomarker, uric acid (UA) is closely associated with normal metabolism and health. Despite versatile nanozymes facilitating the analysis of UA, most previous works could only generate single-signal outputs with unsatisfactory detection performance. Exploring a novel ratiometric fluorescent UA sensor with high sensitivity, reliability and portable sensing ability based on facile, low-cost nanozymes is still challenging. Herein, we report the first metal-organic-framework (MOF) nanozyme-originated ratiometric fluorescent UA sensor based on Fe
3
Ni-MOF-NH
2
propelled UA/uricase/
o
-phenylenediamine tandem catalytic reaction. Different from previous reports, the peroxidase-like property and fluorescence of Fe
3
Ni-MOF-NH
2
were simultaneously employed. In the absence of UA, only the MOF's fluorescence at 430 nm (FI
430
) can be observed, while the addition of UA will initiate UA/uricase catalytic reaction, and the generated H
2
O
2
could oxidize
o
-phenylenediamine into highly fluorescent 2,3-diaminophenazine (DAP) (emission at 565 nm, FI
565
) under the catalysis of the MOF nanozyme. Coincidently, MOF's fluorescence can be quenched by DAP
via
the inner filter effect, resulting in a low FI
430
value and high FI
565
value, respectively. Therefore, H
2
O
2
and UA can be alternatively detected through monitoring the above contrary fluorescence changes. The limit of detection for UA is 24 nM, which is much lower than those in most previous works, and the lowest among nanozyme-based ratiometric fluorescent UA sensors reported to date. Moreover, the portable sensing of UA
via
smartphone-based RGB analysis was facilely achieved by virtue of the above nanozyme-propelled tandem catalytic system, and MOF nanozyme-based molecular contrary logic pairs were further implemented accordingly.
The first metal-organic-framework (MOF) nanozyme-based ratiometric fluorescent sensor for uric acid is constructed. |
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ISSN: | 2040-3364 2040-3372 |
DOI: | 10.1039/d3nr05028a |