Biomimetic mineralization of platelet lysate/oxidized dextran cryogel as a macroporous 3D composite scaffold for bone repair

Scaffold development approaches using autologous sources for tissue repair are of great importance in obtaining bio-active/-compatible constructs. Platelet-rich plasma (PRP) containing various growth factors and platelet lysate (PL) derived from PRP are autologous products that have the potential to...

Full description

Saved in:
Bibliographic Details
Published in:Biomedical materials (Bristol) 2024-03, Vol.19 (2), p.25006
Main Authors: Şeker, Şükran, Aral, Dilara, Elçin, Ayşe Eser, Yaşar Murat, Elçin
Format: Article
Language:English
Subjects:
biomineralization
bone tissue engineering
cryogel scaffold
macroporous scaffolds
oxidized dextran
platelet lysate
platelet rich plasma
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Scaffold development approaches using autologous sources for tissue repair are of great importance in obtaining bio-active/-compatible constructs. Platelet-rich plasma (PRP) containing various growth factors and platelet lysate (PL) derived from PRP are autologous products that have the potential to accelerate the tissue repair response by inducing a transient inflammatory event. Considering the regenerative capacity of PRP and PL, PRP/PL-based scaffolds are thought to hold great promise in tissue engineering by providing an ideal autologous growth factor source and mechanical support for cells. Here, a bio-mineralized PRP-based scaffold was developed using oxidized dextran (OD) and evaluated for future application in bone tissue engineering. Prepared PL/OD scaffolds were incubated in simulated body fluid (SBF) for 7, 14 and 21 day periods. Mineralized PL/OD scaffolds were characterized using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), porosity and compression tests. SEM and EDX analyses revealed mineral accumulation on the PL/OD scaffold as a result of SBF incubation. In vitro cytotoxicity and in vitro hemolysis tests revealed that the scaffolds were non-toxic and hemocompatible. Additionally, human osteoblasts (hOBs) exhibited good attachment and spreading behavior on the scaffolds and maintained their viability throughout the culture period. The alkaline phosphatase activity assay and calcium release results revealed that PL/OD scaffolds preserved the osteogenic properties of hOBs. Overall, findings suggest that mineralized PL/OD scaffold may be a promising scaffold for bone tissue engineering.
ISSN:1748-6041
1748-605X
DOI:10.1088/1748-605X/ad1c9a