How many Mutations are needed to Evolve the Chemical Makeup of a Synthetic Cell?

The chemical evolution of a synthetic cell endowed with a synthetic amino acid as building block, analog to tryptophan, required the emergence of key mutations in genes involved in, inter alia, the general stress response, amino acid metabolism, stringent response, and chemotaxis. Understanding adap...

Full description

Saved in:
Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2024-03, Vol.25 (6), p.e202300829-n/a
Main Authors: Lefèvre‐Morand, Rodrigue Yves Louis, Nikel, Pablo Iván, Acevedo‐Rocha, Carlos G.
Format: Article
Language:English
Subjects:
Adaptative Laboratory Evolution
Amino Acids
Artificial Cells
Cellular stress response
Chemical evolution
Chemotaxis
Metabolic Engineering
Metabolic Networks and Pathways
Metabolic pathways
Mutation
RpoS
Stress Response
Stringent response
Synthetic Biology
Tryptophan
Xenobiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The chemical evolution of a synthetic cell endowed with a synthetic amino acid as building block, analog to tryptophan, required the emergence of key mutations in genes involved in, inter alia, the general stress response, amino acid metabolism, stringent response, and chemotaxis. Understanding adaptation mechanisms to non‐canonical biomass components will inform strategies for engineering synthetic metabolic pathways and cells. Creating new biochemistries in living organisms requires adaptation for learning how to use new resources, a process governed by genetic mutations. When fed with tryptophan analogs, Escherichia coli cells learned to repress their general stress response and other molecular bioprocesses. This discovery creates more opportunities for the incorporation of non‐canonical amino acids with benefits in Xenobiology, Metabolic Engineering and Biocatalysis.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.202300829