Assessment of toxicity, genotoxicity and oxidative stress in Fejervarya limnocharis exposed to tributyltin

• Published in: Environmental science and pollution research international 2024-02, Vol.31 (10), p.14938-14948
• Main Authors: Mandal, Abhijit, Giri, Sarbani, Giri, Anirudha
• Format: Article
• Language: English
• Subjects: Acute toxicity; Amphibians; Animals; Antifouling substances; Anura; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Biocides; DNA Damage; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Exposure; Fejervarya limnocharis; Genotoxicity; Juveniles; Larva; Life history; Metamorphosis; Metamorphosis, Biological; Oxidative Stress; Population decline; Reptiles & amphibians; Research Article; Survivability; Toxicity; Trialkyltin Compounds - toxicity; Tributyltin; Tributyltin compounds; Waste Water Technology; Water Management; Water Pollutants, Chemical - toxicity; Water Pollution Control
• ISSN: 1614-7499; 0944-1344; 1614-7499
• DOI: 10.1007/s11356-024-32220-5

Tributyltin (TBT) is widely used in various commercial applications due to its biocidal properties. Toxicological and genotoxicological data on TBT exposure to amphibians is insufficient. Our study aimed to determine the acute toxicity and genotoxic potential of TBT in Fejervarya limnocharis tadpoles. Furthermore, oxidative stress was also investigated in TBT-treated tadpoles. Tadpoles of Gosner stage (26–30) were screened and subjected to increasing concentrations of TBT (0, 3, 7, 11, 15, 19, 23 µg/L) for determining the LC 50 values for 24 h, 48 h, 72 h, and 96 h. LC 50 values of TBT for 24 h, 48 h, 72 h, and 96 h were found to be 19.45, 15.07, 13.12, and 11.84 μg/L respectively. Based on the 96 h LC 50 value (11.84 µg/L), tadpoles were exposed to different sub-lethal concentrations of TBT for the evaluation of its genotoxic potential and effects on oxidative balance. The role of TBT on survivability, growth, and time to metamorphosis was also assessed. TBT exposure significantly altered the life history traits measured, increased mortality, and delayed the time taken to metamorphosis. Results indicated significant induction of micronucleus (MN, p